Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Mus musculus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P97501 | gene Fmo3; gene FMO3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hepa-1 cell | - |
Mus musculus | - |
Hepa-1c1c7 cell | - |
Mus musculus | - |
hepatocyte | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethionamide + NADPH + H+ + O2 | - |
Mus musculus | ethionamide N-oxide + NADP+ + H2O | - |
? | |
sulindac sulfide + NADPH + H+ + O2 | - |
Mus musculus | sulindac + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavin-containing monooxygenase-3 | - |
Mus musculus |
FMO3 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Mus musculus | |
NADPH | - |
Mus musculus |
Organism | Comment | Expression |
---|---|---|
Mus musculus | no induction of FMO3 in Hepa-1 cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin, DMSO, beta-naphthoflavon, 3,3',4,4',5-pentachlorobiphenyl, butylated hydroxyanisole, menadione, sulphoraphane, and tert-butylhydroquinone | additional information |
Mus musculus | 8fold induction of FMO3 in liver by 3-methylcholanthrene. In Hepa-1 cells, 3-methylcholanthrene and benzo[a]pyrene induce FMO3 mRNA by about 30fold in an aryl hydrocarbon receptor-dependent manner. Aryl hydrocarbon receptor, AHR, dependent induction of FMO mRNAs in liver by 2,3,7,8-tetrachlorodibenzo-p-dioxin, but the potent AHR agonist, TCDD, does not induce FMO3 mRNA in Hepa-1 cells. Mechanism of FMO3 mRNA induction, overview | up |
General Information | Comment | Organism |
---|---|---|
additional information | chromatin immunoprecipitation assays do not detect recruitment of aryl hydrocarbon receptor or ARNT to Fmo3 regulatory elements after exposure to 3-methylcholanthrene in liver or in Hepa-1 cells. However, in Hepa-1, 3-methylcholanthrene and benzo[a]pyrene , but not 2,3,7,8-tetrachlorodibenzo-p-dioxin, cause recruitment of p53 protein to a p53 response element in the 5'-flanking region of the Fmo3 gene. Although FMO3 mRNA is highly induced by 3-methylcholanthrene or 2,3,7,8-tetrachlorodibenzo-p-dioxin in mouse liver and in Hepa-1 cells, FMO protein levels and FMO catalytic function show only modest elevation | Mus musculus |