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Literature summary for 1.14.13.8 extracted from

  • Zhang, J.; Cerny, M.A.; Lawson, M.; Mosadeghi, R.; Cashman, J.R.
    Functional activity of the mouse flavin-containing monooxygenase forms 1, 3, and 5 (2007), J. Biochem. Mol. Toxicol., 21, 206-215.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of maltose-binding protein fusion enzymes FMO1, FMO3, and FMO5 in Escherichia coli Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0032
-
4-(4-methylphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid pH 8.4, 37°C, recombinant FMO5 Mus musculus
0.0038
-
4-(4-methylphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid pH 8.4, 37°C, recombinant FMO1 Mus musculus
0.0068
-
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine pH 8.4, 37°C, recombinant FMO1 Mus musculus
0.00875
-
trimethylamine pH 8.4, 37°C, recombinant FMO3 Mus musculus
0.0245
-
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine pH 8.4, 37°C, recombinant FMO3 Mus musculus
0.0437
-
mercaptoimidazole pH 8.4, 37°C, recombinant FMO3 Mus musculus
0.0527
-
mercaptoimidazole pH 8.4, 37°C, recombinant FMO1 Mus musculus
0.0711
-
4-(4-methoxyphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid pH 8.4, 37°C, recombinant FMO1 Mus musculus
0.0808
-
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine pH 8.4, 37°C, recombinant FMO5 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Mus musculus
-
-

Organism

Organism UniProt Comment Textmining
Mus musculus
-
male C57BL6 mice, isozymes FMO1, FMO3, and FMO5
-

Purification (Commentary)

Purification (Comment) Organism
recombinant maltose-binding protein fusion enzymes FMO1, FMO3, and FMO5 from Escherichia coli by amylose affinity chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver FMO1, FMO3, and FMO5 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Mus musculus
0.0015
-
purified recombinant FMO5, substrate 5-DPT Mus musculus
0.012
-
purified recombinant FMO5, substrate 8-DPT Mus musculus
0.018
-
purified recombinant FMO3, substrate 3-DPT Mus musculus
0.041
-
purified recombinant FMO3, substrate 5-DPT Mus musculus
0.069
-
purified recombinant FMO3, substrate 8-DPT Mus musculus
0.195
-
purified recombinant FMO1, substrate 3-DPT Mus musculus
0.294
-
purified recombinant FMO1, substrate 8-DPT Mus musculus
0.407
-
purified recombinant FMO1, substrate 5-DPT Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetylsulfanylmethyl-4-(4-methoxyphenyl)-4-oxobutyric acid + NADPH + H+ + O2
-
Mus musculus ?
-
?
2-[2-(4-methoxyphenyl)-2-oxoethyl]-acrylic acid + NADPH + H+ + O2 a synthetic 10-(N,N-dimethylaminoalkyl)-2-(trifluoromethyl)phenothiazine analogue Mus musculus ?
-
?
4-(4-methoxyphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid + NADPH + H+ + O2 FMO1, poor activity with FMO3 and FMO5 Mus musculus ?
-
?
4-(4-methylphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid + NADPH + H+ + O2 FMO1 and FMO5, no activity with FMO3 Mus musculus ?
-
?
mercaptoimidazole + NADPH + H+ + O2 FMO1 and FMO3, no activity with FMO5 Mus musculus ?
-
?
N,N-dimethyl-3-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]propan-1-amine + NADPH + H+ + O2 3-DPT, a phenothiazine analogue, N-oxygenation by FMO1 and FMO3, no activity with FMO5 Mus musculus ?
-
?
N,N-dimethyl-5-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]pentan-1-amine + NADPH + H+ + O2 5-DPT, a phenothiazine analogue, N-oxygenation by FMO1, FMO3, and FMO5 Mus musculus ?
-
?
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine + NADPH + H+ + O2 8-DPT, a phenothiazine analogue, N-oxygenation by FMO1, FMO3, and FMO5 Mus musculus ?
-
?
S-methyl esonarimod + NADPH + H+ + O2
-
Mus musculus ?
-
?
trimethylamine + NADPH + H+ + O2 FMO1, no activity with FMO3 and FMO5 Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
FMO1
-
Mus musculus
FMO3
-
Mus musculus
FMO5
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified recombinant FMO1, FMO3, and FMO5, the isozymes display similar thermal sensitivity, with activity decreased to 76% to 83% after 1 min preincubation at 40°C, 61% to 71% after 2 min preincubation, and 41% to 55% after 5 min preincubation Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
assay at Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
7 11 pH profile for the recombinant FMO1, FMO3, and FMO5, overview Mus musculus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Mus musculus
NADPH
-
Mus musculus