Cloned (Comment) | Organism |
---|---|
expression of maltose-binding protein fusion enzymes FMO1, FMO3, and FMO5 in Escherichia coli | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
4-(4-methylphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid | pH 8.4, 37°C, recombinant FMO5 | Mus musculus | |
0.0038 | - |
4-(4-methylphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid | pH 8.4, 37°C, recombinant FMO1 | Mus musculus | |
0.0068 | - |
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine | pH 8.4, 37°C, recombinant FMO1 | Mus musculus | |
0.00875 | - |
trimethylamine | pH 8.4, 37°C, recombinant FMO3 | Mus musculus | |
0.0245 | - |
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine | pH 8.4, 37°C, recombinant FMO3 | Mus musculus | |
0.0437 | - |
mercaptoimidazole | pH 8.4, 37°C, recombinant FMO3 | Mus musculus | |
0.0527 | - |
mercaptoimidazole | pH 8.4, 37°C, recombinant FMO1 | Mus musculus | |
0.0711 | - |
4-(4-methoxyphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid | pH 8.4, 37°C, recombinant FMO1 | Mus musculus | |
0.0808 | - |
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine | pH 8.4, 37°C, recombinant FMO5 | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Mus musculus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
male C57BL6 mice, isozymes FMO1, FMO3, and FMO5 | - |
Purification (Comment) | Organism |
---|---|
recombinant maltose-binding protein fusion enzymes FMO1, FMO3, and FMO5 from Escherichia coli by amylose affinity chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | FMO1, FMO3, and FMO5 | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mus musculus |
0.0015 | - |
purified recombinant FMO5, substrate 5-DPT | Mus musculus |
0.012 | - |
purified recombinant FMO5, substrate 8-DPT | Mus musculus |
0.018 | - |
purified recombinant FMO3, substrate 3-DPT | Mus musculus |
0.041 | - |
purified recombinant FMO3, substrate 5-DPT | Mus musculus |
0.069 | - |
purified recombinant FMO3, substrate 8-DPT | Mus musculus |
0.195 | - |
purified recombinant FMO1, substrate 3-DPT | Mus musculus |
0.294 | - |
purified recombinant FMO1, substrate 8-DPT | Mus musculus |
0.407 | - |
purified recombinant FMO1, substrate 5-DPT | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetylsulfanylmethyl-4-(4-methoxyphenyl)-4-oxobutyric acid + NADPH + H+ + O2 | - |
Mus musculus | ? | - |
? | |
2-[2-(4-methoxyphenyl)-2-oxoethyl]-acrylic acid + NADPH + H+ + O2 | a synthetic 10-(N,N-dimethylaminoalkyl)-2-(trifluoromethyl)phenothiazine analogue | Mus musculus | ? | - |
? | |
4-(4-methoxyphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid + NADPH + H+ + O2 | FMO1, poor activity with FMO3 and FMO5 | Mus musculus | ? | - |
? | |
4-(4-methylphenyl)-2-methylsulfanylmethyl-4-oxobutyric acid + NADPH + H+ + O2 | FMO1 and FMO5, no activity with FMO3 | Mus musculus | ? | - |
? | |
mercaptoimidazole + NADPH + H+ + O2 | FMO1 and FMO3, no activity with FMO5 | Mus musculus | ? | - |
? | |
N,N-dimethyl-3-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]propan-1-amine + NADPH + H+ + O2 | 3-DPT, a phenothiazine analogue, N-oxygenation by FMO1 and FMO3, no activity with FMO5 | Mus musculus | ? | - |
? | |
N,N-dimethyl-5-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]pentan-1-amine + NADPH + H+ + O2 | 5-DPT, a phenothiazine analogue, N-oxygenation by FMO1, FMO3, and FMO5 | Mus musculus | ? | - |
? | |
N,N-dimethyl-8-[2-(trifluoromethyl)-10H-phenothiazin-10-yl]octan-1-amine + NADPH + H+ + O2 | 8-DPT, a phenothiazine analogue, N-oxygenation by FMO1, FMO3, and FMO5 | Mus musculus | ? | - |
? | |
S-methyl esonarimod + NADPH + H+ + O2 | - |
Mus musculus | ? | - |
? | |
trimethylamine + NADPH + H+ + O2 | FMO1, no activity with FMO3 and FMO5 | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FMO1 | - |
Mus musculus |
FMO3 | - |
Mus musculus |
FMO5 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant FMO1, FMO3, and FMO5, the isozymes display similar thermal sensitivity, with activity decreased to 76% to 83% after 1 min preincubation at 40°C, 61% to 71% after 2 min preincubation, and 41% to 55% after 5 min preincubation | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | pH profile for the recombinant FMO1, FMO3, and FMO5, overview | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Mus musculus | |
NADPH | - |
Mus musculus |