Literature summary for 1.14.13.8 extracted from

Shimizu, M.; Yano, H.; Nagashima, S.; Murayama, N.; Zhang, J.; Cashman, J.R.; Yamazaki, H.
Effect of genetic variants of the human flavin-containing monooxygenase 3 on N- and S-oxygenation activities (2007), Drug Metab. Dispos., 35, 328-330.

Search for more literature for 1.14.13.8

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant FMO3 in Escherichia coli strain JM109	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E158K	naturally occuring genetic variant of isozyme FMO3, and site-directed mutagenesis, the mutant shows reduced activity with sulindac and methyl 4-toyl sulfide compared to the wild-type FMO3	Homo sapiens
E158K/E308G	naturally occuring genetic variant of isozyme FMO3, and site-directed mutagenesis, the mutant shows reduced activity with sulindac and methyl 4-toyl sulfide compared to the wild-type FMO3	Homo sapiens
E158K/T201K/E308G	naturally occuring genetic variant of isozyme FMO3, and site-directed mutagenesis, the mutant shows reduced activity with sulindac and methyl 4-toyl sulfide compared to the wild-type FMO3	Homo sapiens
additional information	effects of genetic variants of isozyme FMO3 on N- and S-oxygenation activities, genotype-phenotype studies, overview	Homo sapiens
R205C	naturally occuring genetic variant of isozyme FMO3, and site-directed mutagenesis, the mutant shows reduced activity with sulindac and methyl 4-toyl sulfide compared to the wild-type FMO3, and is almost substrate inhibited, wild-type FMO3 has no free cysteine residues in the native form	Homo sapiens
V257M	naturally occuring genetic variant of isozyme FMO3, and site-directed mutagenesis, the mutant shows reduced activity with sulindac and methyl 4-toyl sulfide compared to the wild-type FMO3	Homo sapiens
V257M/M260V	naturally occuring genetic variant of isozyme FMO3, and site-directed mutagenesis, the mutant shows reduced activity with sulindac and methyl 4-toyl sulfide compared to the wild-type FMO3	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
methyl 4-tolyl sulfide + NADPH + O2	Homo sapiens	-	methyl 4-tolyl sulfoxide + NADP+ + H2O	-	?
additional information	Homo sapiens	effects of genetic variants of isozyme FMO3 on N- and S-oxygenation activities, FMO3 polymorphisms are responsible for the genetic disorder trimethylaminuria, or fish-like odor syndrome, overview	?	-	?
sulindac sulfide + NADPH + O2	Homo sapiens	-	(S,R)-sulindac + NADP+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isozyme FMO3	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant FMO3 from Escherichia coli strain JM109 in a multistep process	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methyl 4-tolyl sulfide + NADPH + O2	-	Homo sapiens	methyl 4-tolyl sulfoxide + NADP+ + H2O	-	?
additional information	effects of genetic variants of isozyme FMO3 on N- and S-oxygenation activities, FMO3 polymorphisms are responsible for the genetic disorder trimethylaminuria, or fish-like odor syndrome, overview	Homo sapiens	?	-	?
sulindac sulfide + NADPH + O2	-	Homo sapiens	(S,R)-sulindac + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
FMO	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.4	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Homo sapiens
NADPH	-	Homo sapiens

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Release 2023.1 (January 2023)