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Literature summary for 1.14.13.8 extracted from

  • Furnes, B.; Schlenk, D.
    Evaluation of xenobiotic N- and S-oxidation by variant flavin-containing monooxygenase 1 (FMO1) enzymes (2004), Toxicol. Sci., 78, 196-203.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D132H KM-value and Vmax of fenthion are nearly identical to the wild-type values, mutant of FMO3 Homo sapiens
H97Q KM-value for methimazole is 3fold higher than wild-type value, Vmax with methimazole is 1.6fold higher than the wild-type value, KM-value for methyl p-tolyl sulfide is 88% of the wild-type value, Vmax with methyl p-tolyl sulfide is 1.4fold higher than the wild-type value, KM-value for imipramine is is nearly identical to the the wild-type value, Vmax with imipramine is 1.3fold higher than the wild-type value, KM-value for fenthion is 94% of the wild-type value, Vmax with fenthion is 1.3fold higher than the wild-type value, mutant of FMO1 Homo sapiens
I303T KM-value for methimazole is 2.3fold higher than wild-type value, Vmax with methimazole is 1.8fold higher than the wild-type value, KM-value for methyl p-tolyl sulfide is 86% of the wild-type value, Vmax with methyl p-tolyl sulfide is 1.8fold higher than the wild-type value, KM-value for imipramine is identical to the the wild-type value, Vmax with imipramine is 1.4fold higher than the wild-type value, KM-value for fenthion is 94% of the wild-type value, Vmax with fenthion is 1.6fold higher than the wild-type value, mutant of FMO1 Homo sapiens
I303V KM-value for methimazole is identical to the wild-type value, Vmax with methimazole is nearly identical to the wild-type value, KM-value and Vmax for meth is 1,4fold higher than the the wild-type value, Vmax with imipramine is nearly identical to the wild-type value, KM-value and Vmax for fenthion are nearly identical to the wild-type values, mutant of FMO1 Homo sapiens
K158L Km-value for fenthion is 1.4fold higher than the wild-type value, Vmax for fenthion is nearly identical to the wild-type value, mutant of FMO3 Homo sapiens
K158L/D132H Km-value for fenthion is 1.5fold higher than the wild-type value, Vmax for fenthion is 1.5fold higher than the wild-type value, mutant of FMO3 Homo sapiens
R502V no activity with methimazole, KM-value for methyl p-tolyl sulfide is 70% of the wild-type value, Vmax with methyl p-tolyl sulfide is 70% of the wild-type value, KM-value for imipramine is is nearly identical to the the wild-type value, Vmax with imipramine is 49% of the wild-type value, KM-value for fenthion is 88% of the wild-type value, Vmax with fenthion is 55% of wild-type value, mutant of FMO1 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
Methimazole mutant enzyme I303V, FMO1 Homo sapiens
0.007
-
Methimazole wild-type enzyme, FMO1 Homo sapiens
0.014
-
Methimazole mutant enzyme H97Q, FMO1 Homo sapiens
0.014
-
imipramine mutant enzyme I303T, FMO1 Homo sapiens
0.014
-
imipramine wild-type enzyme, FMO1 Homo sapiens
0.015
-
imipramine mutant enzyme H97Q, FMO1 Homo sapiens
0.016
-
Methimazole mutant enzyme I303T, FMO1 Homo sapiens
0.016
-
imipramine mutant enzyme R502X, FMO1 Homo sapiens
0.02
-
imipramine mutant enzyme I303V, FMO1 Homo sapiens
0.145
-
fenthion wild-type enzyme, FMO3 Homo sapiens
0.15
-
fenthion mutant enzyme D132H, FMO3 Homo sapiens
0.2
-
fenthion mutant enzyme K158L, FMO3 Homo sapiens
0.2
-
methyl p-tolyl sulfide mutant enzyme R502X, FMO1 Homo sapiens
0.22
-
fenthion mutant enzyme K158L/D132H, FMO3 Homo sapiens
0.24
-
fenthion mutant enzyme I303T, FMO1 Homo sapiens
0.245
-
methyl p-tolyl sulfide mutant enzyme I303T, FMO1 Homo sapiens
0.251
-
methyl p-tolyl sulfide mutant enzyme H97Q, FMO1 Homo sapiens
0.284
-
methyl p-tolyl sulfide wild-type enzyme, FMO1 Homo sapiens
0.3
-
methyl p-tolyl sulfide mutant enzyme I303V, FMO1 Homo sapiens
0.3
-
fenthion mutant enzyme R502X, FMO1 Homo sapiens
0.32
-
fenthion mutant enzyme H97Q, FMO1 Homo sapiens
0.34
-
fenthion wild-type enzyme, FMO1 Homo sapiens
0.351
-
fenthion mutant enzyme I303V, FMO1 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fenthion + NADPH + O2
-
Homo sapiens fenthion sulfoxide + NADP+ 74% (+)-sulfoxide ?
fenthion + NADPH + O2
-
Homo sapiens fenthion sulfoxide + NADP+ + H2O more than 95% (+)-sulfoxide ?
imipramine + NADPH + O2
-
Homo sapiens imipramine N-oxide + NADP+ + H2O
-
?
methimazole + NADPH + O2
-
Homo sapiens N-methylmethimidazole-2-sulfinic acid + NADP+ + H2O
-
?
methyl p-tolyl sulfide + NADPH + O2
-
Homo sapiens methyl p-tolyl sulfoxide + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
flavin-containing monooxygenase 1
-
Homo sapiens
flavin-containing monooxygenase 3
-
Homo sapiens
FMO1
-
Homo sapiens
FMO3
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens