Protein Variants | Comment | Organism |
---|---|---|
D132H | KM-value and Vmax of fenthion are nearly identical to the wild-type values, mutant of FMO3 | Homo sapiens |
H97Q | KM-value for methimazole is 3fold higher than wild-type value, Vmax with methimazole is 1.6fold higher than the wild-type value, KM-value for methyl p-tolyl sulfide is 88% of the wild-type value, Vmax with methyl p-tolyl sulfide is 1.4fold higher than the wild-type value, KM-value for imipramine is is nearly identical to the the wild-type value, Vmax with imipramine is 1.3fold higher than the wild-type value, KM-value for fenthion is 94% of the wild-type value, Vmax with fenthion is 1.3fold higher than the wild-type value, mutant of FMO1 | Homo sapiens |
I303T | KM-value for methimazole is 2.3fold higher than wild-type value, Vmax with methimazole is 1.8fold higher than the wild-type value, KM-value for methyl p-tolyl sulfide is 86% of the wild-type value, Vmax with methyl p-tolyl sulfide is 1.8fold higher than the wild-type value, KM-value for imipramine is identical to the the wild-type value, Vmax with imipramine is 1.4fold higher than the wild-type value, KM-value for fenthion is 94% of the wild-type value, Vmax with fenthion is 1.6fold higher than the wild-type value, mutant of FMO1 | Homo sapiens |
I303V | KM-value for methimazole is identical to the wild-type value, Vmax with methimazole is nearly identical to the wild-type value, KM-value and Vmax for meth is 1,4fold higher than the the wild-type value, Vmax with imipramine is nearly identical to the wild-type value, KM-value and Vmax for fenthion are nearly identical to the wild-type values, mutant of FMO1 | Homo sapiens |
K158L | Km-value for fenthion is 1.4fold higher than the wild-type value, Vmax for fenthion is nearly identical to the wild-type value, mutant of FMO3 | Homo sapiens |
K158L/D132H | Km-value for fenthion is 1.5fold higher than the wild-type value, Vmax for fenthion is 1.5fold higher than the wild-type value, mutant of FMO3 | Homo sapiens |
R502V | no activity with methimazole, KM-value for methyl p-tolyl sulfide is 70% of the wild-type value, Vmax with methyl p-tolyl sulfide is 70% of the wild-type value, KM-value for imipramine is is nearly identical to the the wild-type value, Vmax with imipramine is 49% of the wild-type value, KM-value for fenthion is 88% of the wild-type value, Vmax with fenthion is 55% of wild-type value, mutant of FMO1 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
Methimazole | mutant enzyme I303V, FMO1 | Homo sapiens | |
0.007 | - |
Methimazole | wild-type enzyme, FMO1 | Homo sapiens | |
0.014 | - |
Methimazole | mutant enzyme H97Q, FMO1 | Homo sapiens | |
0.014 | - |
imipramine | mutant enzyme I303T, FMO1 | Homo sapiens | |
0.014 | - |
imipramine | wild-type enzyme, FMO1 | Homo sapiens | |
0.015 | - |
imipramine | mutant enzyme H97Q, FMO1 | Homo sapiens | |
0.016 | - |
Methimazole | mutant enzyme I303T, FMO1 | Homo sapiens | |
0.016 | - |
imipramine | mutant enzyme R502X, FMO1 | Homo sapiens | |
0.02 | - |
imipramine | mutant enzyme I303V, FMO1 | Homo sapiens | |
0.145 | - |
fenthion | wild-type enzyme, FMO3 | Homo sapiens | |
0.15 | - |
fenthion | mutant enzyme D132H, FMO3 | Homo sapiens | |
0.2 | - |
fenthion | mutant enzyme K158L, FMO3 | Homo sapiens | |
0.2 | - |
methyl p-tolyl sulfide | mutant enzyme R502X, FMO1 | Homo sapiens | |
0.22 | - |
fenthion | mutant enzyme K158L/D132H, FMO3 | Homo sapiens | |
0.24 | - |
fenthion | mutant enzyme I303T, FMO1 | Homo sapiens | |
0.245 | - |
methyl p-tolyl sulfide | mutant enzyme I303T, FMO1 | Homo sapiens | |
0.251 | - |
methyl p-tolyl sulfide | mutant enzyme H97Q, FMO1 | Homo sapiens | |
0.284 | - |
methyl p-tolyl sulfide | wild-type enzyme, FMO1 | Homo sapiens | |
0.3 | - |
methyl p-tolyl sulfide | mutant enzyme I303V, FMO1 | Homo sapiens | |
0.3 | - |
fenthion | mutant enzyme R502X, FMO1 | Homo sapiens | |
0.32 | - |
fenthion | mutant enzyme H97Q, FMO1 | Homo sapiens | |
0.34 | - |
fenthion | wild-type enzyme, FMO1 | Homo sapiens | |
0.351 | - |
fenthion | mutant enzyme I303V, FMO1 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fenthion + NADPH + O2 | - |
Homo sapiens | fenthion sulfoxide + NADP+ | 74% (+)-sulfoxide | ? | |
fenthion + NADPH + O2 | - |
Homo sapiens | fenthion sulfoxide + NADP+ + H2O | more than 95% (+)-sulfoxide | ? | |
imipramine + NADPH + O2 | - |
Homo sapiens | imipramine N-oxide + NADP+ + H2O | - |
? | |
methimazole + NADPH + O2 | - |
Homo sapiens | N-methylmethimidazole-2-sulfinic acid + NADP+ + H2O | - |
? | |
methyl p-tolyl sulfide + NADPH + O2 | - |
Homo sapiens | methyl p-tolyl sulfoxide + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavin-containing monooxygenase 1 | - |
Homo sapiens |
flavin-containing monooxygenase 3 | - |
Homo sapiens |
FMO1 | - |
Homo sapiens |
FMO3 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |