Cloned (Comment) | Organism |
---|---|
co-expression of phenol hydroxylase with the regulatory protein in Escherichia coli JM109, expression of selenomethionine-enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas sp. |
Crystallization (Comment) | Organism |
---|---|
native and SeMet forms of the phenol hydroxylase in complex with its regulatory protein, hanging drop vapor diffusion method, 20°C, 0.035 mM enzyme in 10 mM MES, pH 7.1, and 10% glycerol is mixed with an equal volume of crystallization buffer containing 100 mM Tris, pH 7.0, 150 mM Na2MoO4, 5% glycerol, and 17-20% PEG 8000 (w/w), X-ray diffraction structure determination and analysis at 2.3 Å resolution, molecular replacement, Single-wavelength anomalous dispersion data for the selenomethionine derivative | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains dinuclear iron active site, structure with a Fe(II)Fe(III) oxidation state, helix E comprises part of the iron-coordinating four-helix bundle | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. OX1 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phenol + NADPH + H+ + O2 = catechol + NADP+ + H2O | catalytic mechanism, conformational changes upon binding of the regulatory protein comonent and effects on catalytic activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is a hydrocarbon-oxidizing multicomponent monooxygenase, important for activity is formation of a complex between the hydroxylase and a regulatory protein component | Pseudomonas sp. | ? | - |
? | |
additional information | the enzyme is a hydrocarbon-oxidizing multicomponent monooxygenase, important for activity is formation of a complex between the hydroxylase and a regulatory protein component | Pseudomonas sp. OX1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the regulatory protein component binds in a canyon on one side of the (alpha,beta)2 enzyme dimer, contacting alpha-subunit helices A, E, and F about 12 A above the diiron core | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
phenol hydroxylase | - |
Pseudomonas sp. |
PHH | - |
Pseudomonas sp. |