Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.7 extracted from

  • Waters, S.; Neujahr, H.Y.
    Sources and nature of heterogeneity in recombinant phenol hydroxylase derived from the basidiomycetous soil yeast Trichosporon cutaneum (1997), Biotechnol. Appl. Biochem., 25, 235-242.
No PubMed abstract available
Search for more literature for 1.14.13.7

Cloned(Commentary)

Cloned (Comment) Organism
-
 Cutaneotrichosporon cutaneum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 4 * 76000, non-denaturing PAGE, after expression in Escherichia coli Cutaneotrichosporon cutaneum
302000
-
 gel filtration Cutaneotrichosporon cutaneum

Organism

Organism UniProt Comment Textmining
Cutaneotrichosporon cutaneum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.84
-
 tetrameric form Cutaneotrichosporon cutaneum
1.5
-
 reconstituted tetrameric form Cutaneotrichosporon cutaneum
2.3
-
 dimeric form Cutaneotrichosporon cutaneum
2.6
-
 reconstituted dimeric form Cutaneotrichosporon cutaneum

Subunits

Subunits Comment Organism
tetramer 4 * 76000, non-denaturing PAGE, after expression in Escherichia coli Cutaneotrichosporon cutaneum

Cofactor

Cofactor Comment Organism Structure
FAD 2 FAD per dimer and 3 FAD per tetramer after removing FAD and reconstituting the apoenzyme with the cofactor Cutaneotrichosporon cutaneum