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Literature summary for 1.14.13.69 extracted from

  • Fosdike, W.L.; Smith, T.J.; Dalton, H.
    Adventitious reactions of alkene monooxygenase reveal common reaction pathways and component interactions among bacterial hydrocarbon oxygenases (2005), FEBS J., 272, 2661-2669.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
H2O2
-
Rhodococcus rhodochrous

Inhibitors

Inhibitors Comment Organism Structure
Ethyne irreversible inhibition in the presence of NADH, suicide substrate, growth inhibition when added to the culture medium Rhodococcus rhodochrous
propyne irreversible inhibition in the presence of NADH, suicide substrate, growth inhibition when added to the culture medium Rhodococcus rhodochrous

Metals/Ions

Metals/Ions Comment Organism Structure
Iron binuclear non-heme iron center Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
propene + NADH + O2 Rhodococcus rhodochrous enables the growth on propene as sole carbon and energy source 1,2-epoxypropane + NAD+ + H2O
-
?
propene + NADH + O2 Rhodococcus rhodochrous B-276 enables the growth on propene as sole carbon and energy source 1,2-epoxypropane + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
formerly Nocardia corallina, high expression of enzyme when grown with propene as sole carbon and energy source
-
Rhodococcus rhodochrous B-276
-
formerly Nocardia corallina, high expression of enzyme when grown with propene as sole carbon and energy source
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodococcus rhodochrous

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.145
-
purified enzyme, pH 7.5, 30°C Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propene + NADH + O2
-
Rhodococcus rhodochrous 1,2-epoxypropane + NAD+ + H2O
-
?
propene + NADH + O2 enables the growth on propene as sole carbon and energy source Rhodococcus rhodochrous 1,2-epoxypropane + NAD+ + H2O
-
?
propene + NADH + O2
-
Rhodococcus rhodochrous B-276 1,2-epoxypropane + NAD+ + H2O
-
?
propene + NADH + O2 enables the growth on propene as sole carbon and energy source Rhodococcus rhodochrous B-276 1,2-epoxypropane + NAD+ + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
NADH
-
Rhodococcus rhodochrous