Cloned (Comment) | Organism |
---|---|
gene aerA, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and P14G mutant enzymes in strain M15-2, comparison of nucleotide sequences of enzyme-encoding genes iucD and aerA | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
P14G | site-directed mutagenesis, requires different reaction conditions for full activity and shows altered cofactor specificity than the wild-type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FAD | inhibition of the wild-type and mutant P14G enzymes at very high concentrations | Escherichia coli | |
L-lysine | substrate inhibition of the wild-type and mutant P14G enzymes | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene aerA plasmid-encoded | - |
Escherichia coli EN222 | - |
gene aerA plasmid-encoded | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and P14G mutant enzymes from strain M15-2 to homogeneity by gel filtration, ion exchange and nickel affinity chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00016 | - |
recombinant mutant P14G, with 0.005 mM FAD, and NADPH | Escherichia coli |
0.0011 | - |
recombinant mutant P14G, with 0.005 mM FAD, and iodine | Escherichia coli |
0.0039 | - |
recombinant mutant P14G, with 0.3 mM FAD, and NADPH | Escherichia coli |
0.028 | - |
recombinant mutant P14G, with 0.3 mM FAD, and iodine | Escherichia coli |
0.3 | - |
recombinant wild-type enzyme, with 0.005 mM FAD, and iodine | Escherichia coli |
0.444 | - |
recombinant wild-type enzyme, with 0.005 mM FAD, and NADPH | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + iodine + O2 | iodine less effective than NADPH for the wild-type enzyme | Escherichia coli | N6-hydroxy-L-lysine + iodate + H2O | - |
? | |
L-lysine + iodine + O2 | iodine less effective than NADPH for the wild-type enzyme | Escherichia coli EN222 | N6-hydroxy-L-lysine + iodate + H2O | - |
? | |
L-lysine + NADPH + O2 | NADPH preferred compared to iodine for the wild-type enzyme | Escherichia coli | N6-hydroxy-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + NADPH + O2 | NADPH preferred compared to iodine for the wild-type enzyme | Escherichia coli EN222 | N6-hydroxy-L-lysine + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | comparison of amino acid sequences of enzyme-encoding genes iucD and aerA | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
LH | - |
Escherichia coli |
Lysine N6-hydroxylase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at with NADPH as cofactor | Escherichia coli |
37 | - |
assay at with iodine as cofactor | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
P14G mutant | Escherichia coli |
8 | - |
wild-type enzyme | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-profile of wild-type and mutant P14G enzymes | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | binding site includes an unusual proline residue | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Escherichia coli |