Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Pregnene-3beta,20alpha-diol | lactonization of androstenedione | Ilyonectria destructans | |
pregnenolone | and other C21-20-ketosteroids which are the substrates for oxygenative esterification of the enzyme, strongly inhibit lactonization of androstenedione | Ilyonectria destructans | |
progesterone | strong, competitive inhibitor of lactonization of 17-ketosteroids | Ilyonectria destructans | |
testosterone | lactonization of androstenedione | Ilyonectria destructans | |
Testosterone acetate | lactonization of androstenedione | Ilyonectria destructans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
progesterone | - |
Ilyonectria destructans | |
0.002 | - |
NADPH | with androstenedione and O2 as cosubstrates | Ilyonectria destructans | |
0.0043 | - |
NADPH | with progesterone and O2 as cosubstrates | Ilyonectria destructans | |
0.04 | - |
androstenedione | - |
Ilyonectria destructans | |
0.07 | - |
O2 | with progesterone and NADPH as cosubstrates | Ilyonectria destructans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ilyonectria destructans | - |
ATTC 11011 | - |
Ilyonectria destructans ATTC 11011 | - |
ATTC 11011 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Ilyonectria destructans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxypregnenolone + NADPH + O2 | - |
Ilyonectria destructans | ? | - |
? | |
17alpha-hydroxypregnenolone + NADPH + O2 | - |
Ilyonectria destructans ATTC 11011 | ? | - |
? | |
17alpha-Hydroxyprogesterone + NADPH + O2 | the 17-ketosteroid acts first with the enzyme, and then NADPH | Ilyonectria destructans | androstenedione + acetate + NADP+ + H2O | 4-androstene-3,17-dione | ? | |
17alpha-Hydroxyprogesterone + NADPH + O2 | the 17-ketosteroid acts first with the enzyme, and then NADPH | Ilyonectria destructans ATTC 11011 | androstenedione + acetate + NADP+ + H2O | 4-androstene-3,17-dione | ? | |
androstenedione + NADPH + O2 | - |
Ilyonectria destructans | 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O | i.e. testololactone | ? | |
androstenedione + NADPH + O2 | - |
Ilyonectria destructans ATTC 11011 | 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O | i.e. testololactone | ? | |
Pregnenolone + NADPH + O2 | - |
Ilyonectria destructans | ? | - |
? | |
Pregnenolone + NADPH + O2 | - |
Ilyonectria destructans ATTC 11011 | ? | - |
? | |
progesterone + NADPH + H+ + O2 | - |
Ilyonectria destructans | Testosterone acetate + NADP+ + H2O | - |
? | |
progesterone + NADPH + H+ + O2 | - |
Ilyonectria destructans ATTC 11011 | Testosterone acetate + NADP+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
progesterone monooxygenase activity | Ilyonectria destructans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
half-life: 8 days | Ilyonectria destructans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
androstenedione monooxygenase reaction | Ilyonectria destructans |
7.8 | - |
progesterone monooxygenase reaction | Ilyonectria destructans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | pH 5.5: about 20% of maximal activity, pH 8.0: about 40% of maximal activity, androstenedione monooxygenase activity | Ilyonectria destructans |