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Literature summary for 1.14.13.54 extracted from

  • Morii, S.; Sawamoto, S.; Yamauchi, Y.; Miyamoto, M.; Iwami, M.; Itagaki, E.
    Steroid monooxygenase of Rhodococcus rhodochrous: sequencing of the genomic DNA, and hyperexpression, purification, and characterization of the recombinant enzyme (1999), J. Biochem., 126, 624-631.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Rhodococcus rhodochrous

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00044
-
NADPH pH 8.3, 37°C Rhodococcus rhodochrous
0.055
-
progesterone pH 8.3, 37°C Rhodococcus rhodochrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Rhodococcus rhodochrous
60133
-
x * 60133, calculated from the deduced amino acid sequence Rhodococcus rhodochrous
60919
-
x * 60919, holoenzyme, calculated from the deduced amino acid sequence Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
progesterone + NADPH + H+ + O2 Rhodococcus rhodochrous
-
Testosterone acetate + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
starin IFO 2228
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Rhodococcus rhodochrous

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.95
-
purified enzyme Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
progesterone + NADPH + H+ + O2
-
Rhodococcus rhodochrous Testosterone acetate + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Rhodococcus rhodochrous
? x * 60133, calculated from the deduced amino acid sequence Rhodococcus rhodochrous
? x * 60919, holoenzyme, calculated from the deduced amino acid sequence Rhodococcus rhodochrous

Cofactor

Cofactor Comment Organism Structure
FAD
-
Rhodococcus rhodochrous
NADPH
-
Rhodococcus rhodochrous