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Literature summary for 1.14.13.51 extracted from

  • Williams, D.R.; Trudgill, P.W.; Taylor, D.G.
    Metabolism of 1,8-cineole by a Rhodococcus species: Ring cleavage reactions (1989), J. Gen. Microbiol., 135, 1957-1967.
No PubMed abstract available
Search for more literature for 1.14.13.51

General Stability

General Stability Organism
5-10 vol% ethanol stabilizes the crude cell extract Rhodococcus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-oxocineole + NADPH + O2 Rhodococcus grown on 1,8-cineole or succinate as carbon source 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one ?
6-oxocineole + NADPH + O2 Rhodococcus C1 grown on 1,8-cineole or succinate as carbon source 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one ?

Organism

Organism UniProt Comment Textmining
Rhodococcus
-
-
-
Rhodococcus C1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rhodococcus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.001
-
 succinate grown cells Rhodococcus
0.3 0.4 1,8-cineole-grown cells Rhodococcus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-oxocineole + NADPH + O2
-
 Rhodococcus 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one ?
6-oxocineole + NADPH + O2 grown on 1,8-cineole or succinate as carbon source Rhodococcus 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one ?
6-oxocineole + NADPH + O2
-
 Rhodococcus C1 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one ?
6-oxocineole + NADPH + O2 grown on 1,8-cineole or succinate as carbon source Rhodococcus C1 1,6,6-trimethyl-2,7-dioxabicyclo-[3.2.2]nonan-3-one + NADP+ + H2O this product reacts spontaneously to 3-(1-hydroxy-1-methylethyl)-6-oxoheptanoic acid and subsequently lactonizes to 4,5-dihydro-5,5-dimethyl-4-(3'-oxobutyl)-furan-2(3H)-one ?
additional information no substrates: substrate analogues, i.e. (+)-camphor, (S)-(+)-carvone, cyclohexanene, 1,3-cyclohexanedione, dihydrocarvone, 2-, 3-, and 4-methylcyclohexanone, fenchone, menthone, pinan-3-one, pulegone, verbenone Rhodococcus ?
-
 ?
additional information no substrates: substrate analogues, i.e. (+)-camphor, (S)-(+)-carvone, cyclohexanene, 1,3-cyclohexanedione, dihydrocarvone, 2-, 3-, and 4-methylcyclohexanone, fenchone, menthone, pinan-3-one, pulegone, verbenone Rhodococcus C1 ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Rhodococcus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Rhodococcus
NADPH
-
 Rhodococcus