Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sphingobium chlorophenolicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
Pentachlorophenol | below 0.001, pH 7.0, 22°C | Sphingobium chlorophenolicum | |
0.0056 | - |
2,3,5,6-Tetrachlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum | |
0.0126 | - |
2,6-dichlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum | |
0.059 | - |
3,4,5-trichlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum | |
0.098 | - |
3,5-dichlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3,5,6-tetrachlorophenol + 2 NADPH + H+ + O2 | - |
Sphingobium chlorophenolicum | 2,3,5,6-tetrachloro-p-hydroquinone + 2 NADP+ + chloride + H2O | - |
? | |
2,6-dichlorophenol + NADPH + H+ + O2 | - |
Sphingobium chlorophenolicum | 2,6-dichloro-p-hydroquinone + NADP+ + H2O | - |
? | |
3,4,5-trichlorophenol + 2 NADPH + H+ + O2 | - |
Sphingobium chlorophenolicum | 3,5-dichloro-p-hydroquinone + 2 NADP+ + chloride + H2O | - |
? | |
3,5-dichlorophenol + NADPH + H+ + O2 | - |
Sphingobium chlorophenolicum | 3,5-dichloro-p-hydroquinone + NADP+ + H2O | - |
? | |
additional information | enzyme exhibits substantial uncoupling. The C4a-hydroxyflavin intermediate, instead of hydroxylating the substrate, can decompose to produce H2O2 in a futile cycle that consumes NADPH. The extent of uncoupling varies from 0 to 100% with different substrates. The extent of uncoupling is increased by the presence of bulky substituents at position 3, 4, or 5 and decreased by the presence of a chlorine in the ortho position. The effectiveness of pentachlorophenol hydroxylase is additionally hindered by its promiscuous activity with tetrachlorohydroquinone, a downstream metabolite in the degradation pathway. The conversion of tetrachlorohydroquinone to tetrachlorobenzoquinone reverses flux through the pathway. Substantial uncoupling also occurs during the reaction with tetrachlorohydroquinone | Sphingobium chlorophenolicum | ? | - |
? | |
pentachlorophenol + 2 NADPH + H+ + O2 | - |
Sphingobium chlorophenolicum | tetrachlorobenzoquinone + 2 NADP+ + chloride + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PCP hydroxylase | - |
Sphingobium chlorophenolicum |
pentachlorophenol hydroxylase | - |
Sphingobium chlorophenolicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
3,5-dichlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum | |
0.01 | - |
3,4,5-trichlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum | |
0.024 | - |
Pentachlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum | |
0.046 | - |
2,6-dichlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum | |
0.32 | - |
2,3,5,6-Tetrachlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.102 | - |
3,5-dichlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum | |
0.169 | - |
3,4,5-trichlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum | |
3.81 | - |
2,6-dichlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum | |
24 | - |
Pentachlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum | |
57 | - |
2,3,5,6-Tetrachlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum |