General Stability | Organism |
---|---|
very unstable, polyols, non-ionic detergents and EDTA have stabilizing effects | Aspergillus terreus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Aspergillus terreus | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
questin + NADPH + H+ + O2 | Aspergillus terreus | absolute requirement for oxygen and NADPH | demethylsulochrin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, additional functional protein or proteins required for full activity | Aspergillus terreus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus terreus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
crude extract | Aspergillus terreus |
Storage Stability | Organism |
---|---|
cold room, 0.01 M Tris-HCl, pH 7.5, 50% ethylene glycol, v/v, 1 mM EDTA, 0.05% Tween 80, w/v, 70-80% of its original activity after 2 days | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
questin + NADPH + H+ + O2 | absolute requirement for oxygen and NADPH | Aspergillus terreus | demethylsulochrin + NADP+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
complete loss of activity overnight | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Aspergillus terreus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | highly specific | Aspergillus terreus |