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Literature summary for 1.14.13.39 extracted from

  • Li, H.; Jamal, J.; Chreifi, G.; Venkatesh, V.; Abou-Ziab, H.; Poulos, T.L.
    Dissecting the kinetics of the NADP(+)-FADH2 charge transfer complex and flavin semiquinones in neuronal nitric oxide synthase (2013), J. Inorg. Biochem., 124, 1-10.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
DELTAG810 deletion changes redox behaviour of mutant. In the early stage of flavin reduction, similar to the case of wild-type, the hydroquinone FADH2-FMN quickly converts to the disemiquinone and does not accumulate. Since more FADH2-FMN is generated and not consumed quickly enough, the decreased flavin absorption band of FADH2-FMN will blur the isosbestic point after 100 ms, most likely due to a slower two-electron reduction of FMN in the mutant Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
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Source Tissue

Source Tissue Comment Organism Textmining

Cofactor

Cofactor Comment Organism Structure
FAD electron flow within the neuronal nitric oxide synthase reductase domain includes hydride transfer from NADPH to FAD followed by two one-electron transfer reactions from FAD to FMN. Binding of the second NADPH is necessary to drive the full reduction of FMN and charge transfer and the subsequent interflavin electron transfer have distinct spectral features that can be monitored separately with stopped flow spectroscopy. Interflavin electron transfer reported at 600 nm is not limiting in nitric oxide synthase catalysis Rattus norvegicus
NADPH electron flow within the neuronal nitric oxide synthase reductase domain includes hydride transfer from NADPH to FAD followed by two one-electron transfer reactions from FAD to FMN. Binding of the second NADPH is necessary to drive the full reduction of FMN and charge transfer and the subsequent interflavin electron transfer have distinct spectral features that can be monitored separately with stopped flow spectroscopy. Interflavin electron transfer reported at 600 nm is not limiting in nitric oxide synthase catalysis Rattus norvegicus