Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the autoinhibitory domain doubles the maximum calmodulin-dependent enzyme activity and decreases the EC50(Ca2+) values for calmodulin binding and calmodulin-dependent enzyme activation | Bos taurus |
S617D | the phosphomimetic substitution doubles the maximum calmodulin-dependent enzyme activity and decreases the EC50(Ca2+) values for calmodulin binding and enzyme activation compared to the wild type enzyme | Bos taurus |
S617D/S635D | the maximum calmodulin-dependent synthase activity of S617/635D eNOS is about 2fold higher than the wild type activity | Bos taurus |
S635D | the substitution has little or no effect on enzyme activity | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for calmodulin to bind and activate the enzyme, maximum calmodulin-dependent activity is measured at 1.5 mM CaCl2 | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P29473 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA Sepharose column chromatography and 2'-5' ADP Sepharose resin column chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
-85°C, 50 mM Tris (pH 7.4) with 10% (v/v) glycerol, 100 mM NaCl, 0.2 mM L-arginine, 0.0002 mM 5,6,7,8-tetrahydro-L-biopterin, 0.02 mM dithiothreitol, and 1 mM 2',3'-AMP (mixed isomers), up to 1 month, no detectable loss of catalytic activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Bos taurus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
oxidized cytochrome c + NADPH + O2 | - |
Bos taurus | reduced cytochrome c + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endothelial nitric oxide synthase | - |
Bos taurus |
eNOS | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | - |
Bos taurus | |
Calmodulin | maximum calmodulin-dependent activity is measured at 1.5 mM CaCl2, phosphorylation within an autoinhibitory domain in endothelial nitric oxide synthase reduces the Ca2+ concentrations required for calmodulin to bind and activate the enzyme | Bos taurus | |
NADPH | - |
Bos taurus |