Literature summary for 1.14.13.39 extracted from

Wei, C.C.; Wang, Z.Q.; Tejero, J.; Yang, Y.P.; Hemann, C.; Hille, R.; Stuehr, D.J.
Catalytic reduction of a tetrahydrobiopterin radical within nitric-oxide synthase (2008), J. Biol. Chem., 283, 11734-11742.

Search for more literature for 1.14.13.39

Cloned(Commentary)

Cloned (Comment)	Organism
expression of nNOS in Escherichia coli	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics, rapid-freeze method, overview	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	dependent on	Rattus norvegicus
Fe2+	a heme enzyme	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 L-arginine + 3 NADPH + 3 H+ + 4 O2	Rattus norvegicus	-	2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P29476	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant nNOS from Escherichia coli in absence of tetrahydrobiopterin	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
neuron	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 L-arginine + 2 NADPH + 2 H+ + 2 O2	first half reaction via intermediate Nomega-hydroxy-L-arginine	Rattus norvegicus	2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O	-	?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2	-	Rattus norvegicus	2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O	-	?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2	complete reaction, during Arg hydroxylation, H4B acts as a one-electron donor and is then presumed to redox cycle, i.e. be reduced back to H4B, within NOS before further catalysis can proceed. Calmodulin-dependent reduction of a tetrahydrobiopterin radical, mechanism involving the NOS flavoprotein domain, reaction scheme, overview	Rattus norvegicus	2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O	-	?
additional information	nitric-oxide synthases are catalytically self-sufficient flavo-heme enzymes that generate NO from L-arginine and display an utilization of their tetrahydrobiopterin cofactor, overview	Rattus norvegicus	?	-	?
Nomega-hydroxy-L-arginine + NADPH + H+ + O2	second half reaction via intermediate Nomega-hydroxy-L-arginine	Rattus norvegicus	citrulline + nitric oxide + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
neuronal NOS	-	Rattus norvegicus
NOS	-	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
Calmodulin	dependent on	Rattus norvegicus
FMNH2	-	Rattus norvegicus
heme	-	Rattus norvegicus
NADPH	-	Rattus norvegicus
tetrahydrobiopterin	-	Rattus norvegicus

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Release 2023.1 (January 2023)