Cloned (Comment) | Organism |
---|---|
overexpression of His6-tagged bNOS in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
recombinant expression of bNOS | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of NO and substrate binding, recombinant enzyme | Staphylococcus aureus | |
additional information | - |
additional information | kinetics of NO and substrate binding, recombinant enzyme | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a heme enzyme, spectral comparison of the FeIII-NO and FeII-NO complexes of the bacterial NOSs, FeIII-NO complexes lack change in Fe-N-O frequencies upon (6R) 5,6,7,8-tetrahydro-L-biopterin binding to bacterial NOSs, overview. In the FeIII-NO complexes, both L-arginine and NOHA induced the Fe-N-O bending mode at nearly the same frequency as a result of a steric interaction between the substrates and the heme-bound NO, while in FeII-NO complexes the the Fe-N-O bending mode is no observed | Staphylococcus aureus | |
Fe2+ | a heme enzyme, spectral comparison of the FeIII-NO and FeII-NO complexes of the bacterial NOSs, FeIII-NO complexes lack change in Fe-N-O frequencies upon (6R) 5,6,7,8-tetrahydro-L-biopterin binding to bacterial NOSs, overview. In the FeIII-NO complexes, both L-arginine and NOHA induced the Fe-N-O bending mode at nearly the same frequency as a result of a steric interaction between the substrates and the heme-bound NO, while in FeII-NO complexes the the Fe-N-O bending mode is no observed | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Staphylococcus aureus | - |
2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Bacillus subtilis | - |
2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant bNOS by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Staphylococcus aureus |
recombinant His6-tagged bNOS from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 2 NADPH + 2 H+ + 2 O2 | first half reaction via intermediate Nomega-hydroxy-L-arginine | Staphylococcus aureus | 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O | - |
? | |
2 L-arginine + 2 NADPH + 2 H+ + 2 O2 | first half reaction via intermediate Nomega-hydroxy-L-arginine | Bacillus subtilis | 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Staphylococcus aureus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Bacillus subtilis | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | overall reaction, the interactions between heme-bound NO and the substrates are finely tuned by the geometry of the Fe-ligand structure, overview | Staphylococcus aureus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | overall reaction, the interactions between heme-bound NO and the substrates are finely tuned by the geometry of the Fe-ligand structure, overview | Bacillus subtilis | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
Nomega-hydroxy-L-arginine + NADPH + H+ + O2 | second half reaction via intermediate Nomega-hydroxy-L-arginine | Bacillus subtilis | citrulline + nitric oxide + NADP+ + H2O | - |
? | |
Nomega-hydroxy-L-arginine + NADPH + H+ + O2 | second half reaction via intermediate Nomega-hydroxy-L-arginine, FeII and FeII-NO complexes bind Nomega-hydroxy-L-arginine, overview | Staphylococcus aureus | citrulline + nitric oxide + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bacterial nitric oxide synthase | - |
Staphylococcus aureus |
bacterial nitric oxide synthase | - |
Bacillus subtilis |
bNOS | - |
Staphylococcus aureus |
bNOS | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme b | bound, quantitative determination | Staphylococcus aureus | |
heme b | bound, quantitative determination | Bacillus subtilis | |
NADPH | - |
Staphylococcus aureus | |
NADPH | - |
Bacillus subtilis |