Cloned (Comment) | Organism |
---|---|
neuronal enzyme, stable expression in HEK-293 cells | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ngamma-nitro-L-arginine methyl ester | nearly complete inhibition at 0.5 mM | Rattus norvegicus | |
PIN | human protein enzyme inhibitor, recombinantly expressed in Escherichia coli, the recombinant CREB-binding protein-bound inhibitor protein is purified by calmodulin affinity and inhibits the enzyme to a high extent at 0.001 mM | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Rattus norvegicus | - |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant neuronal enzyme from HEK-293 cells by 2',5'-ADP affinity chromatography to about 90% purity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neuron | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neuronal nitric oxide synthase | - |
Rattus norvegicus |
nNOS | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus norvegicus |