Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Bos taurus |
Cloned (Comment) | Organism |
---|---|
expression of endothelial isoform in human embryonic kidney cells | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibits at concentrations above 0.01 mM | Bos taurus | |
Ngamma-nitro-L-arginine methyl ester | complete inhibition | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
L-arginine | - |
Bos taurus | |
0.019 | - |
Ngamma-hydroxy-L-arginine | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Bos taurus | |
Iron | heme-iron | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
endothelial isoform | - |
Purification (Comment) | Organism |
---|---|
recombinant endothelial isoform from human embryonic kidney cells | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endothelium | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.143 | - |
purified recombinant enzyme | Bos taurus |
0.41 | - |
substrate Ngamma-hydroxy-L-arginine, purified enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Bos taurus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
ferricyanide + NADPH + O2 | - |
Bos taurus | ferrocyanide + NADP+ + ? | - |
? | |
Ngamma-hydroxy-L-arginine + NADPH + O2 | - |
Bos taurus | citrulline + NADP+ + NO | - |
? | |
oxidized cytochrome c + NADPH + O2 | - |
Bos taurus | reduced cytochrome c + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 130000-150000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
e-NOS | endothelial isoform III | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | - |
Bos taurus | |
Calmodulin | enzyme-bound is required, supplemented stimulates | Bos taurus | |
FAD | - |
Bos taurus | |
FMN | - |
Bos taurus |