Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.39 extracted from

  • Wever, R.M.F.; Van Dam, T.; Van Rijn, H.J.M.; De Groot, F.; Rabelink, T.J.
    Tetrahydrobiopterin regulates superoxide and nitric oxide generation by recombinant endothelial nitric oxide synthase (1997), Biochem. Biophys. Res. Commun., 237, 340-344.
    View publication on PubMed
Search for more literature for 1.14.13.39

Cloned(Commentary)

Cloned (Comment) Organism
expression of endothelial isoform III in Spodoptera frugiperda cells via baculovirus infection Bos taurus

General Stability

General Stability Organism
tetrahydrobiopterin stabilizes dimeric form Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
diphenylene iodonium inhibition of superoxide production of recombinant isoform III Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ Bos taurus
-
 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
additional information Bos taurus enzyme shows also superoxide formation activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant endothelial isoform III from insect cells Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
endothelium isoform III Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Bos taurus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
additional information enzyme shows also superoxide formation activity, uneffected by L-arginine, inhibited by tetrahydrobiopterin and diphenyleneiodonium Bos taurus ?
-
 ?
additional information enzyme shows also superoxide formation activity Bos taurus ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin stimulates Bos taurus
FAD major source of superoxide production in absence of tetrahydrobiopterin Bos taurus
NADPH
-
 Bos taurus