Literature summary for 1.14.13.39 extracted from

Nam, S.W.; Seo, D.W.; Sung, D.S.; Han, J.W.; Hong, S.Y.; Lee, H.W.
Nitric oxide synthase from bovine pancreas: purification and characterization (1998), Arch. Pharm. Res., 21, 128-134.

Search for more literature for 1.14.13.39

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	Ngamma,Ngamma'-dimethyl-L-arginine has no inhibitory effect	Bos taurus
Ngamma,Ngamma-dimethyl-L-arginine	-	Bos taurus
Ngamma-monomethyl-L-arginine	-	Bos taurus
Ngamma-nitro-L-arginine	-	Bos taurus
Ngamma-nitro-L-arginine methyl ester	-	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	0.018	L-arginine	-	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	absolutely dependent on	Bos taurus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
160000	-	2 * 160000, SDS-PAGE	Bos taurus
320000	-	gel filtration	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.031	-	purified pancreatic enzyme	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+	-	Bos taurus	2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 160000, SDS-PAGE	Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	-	Bos taurus

Cofactor

Cofactor	Comment	Organism	Structure
5,6,7,8-tetrahydro-L-biopterin	not required for activity	Bos taurus
Calmodulin	required	Bos taurus
FAD	required	Bos taurus
FMN	required	Bos taurus
NADPH	-	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0015	-	Ngamma-nitro-L-arginine	-	Bos taurus
0.0034	-	Ngamma,Ngamma-dimethyl-L-arginine	-	Bos taurus
0.0054	-	Ngamma-monomethyl-L-arginine	-	Bos taurus
0.0155	-	Ngamma-nitro-L-arginine methyl ester	-	Bos taurus

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Release 2023.1 (January 2023)