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Literature summary for 1.14.13.38 extracted from
- Partial purification and characterization of anhydrotetracycline oxygenase of Streptomyces aureofaciens (1987), J. Basic Microbiol., 27, 529-533.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | addition of supernatant of Streptomyces aureofaciens or Streptomyces rimosus crude extract after boiling and centrifugation increases activity 7fold | Kitasatospora aureofaciens |
General Stability
| General Stability | Organism |
|---|---|
| loss of activity during purification | Kitasatospora aureofaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiobis-(2-nitrobenzoic acid) | 40% inhibition at 1 mM, irreversible | Kitasatospora aureofaciens |  |
| iodoacetamide | 14% inhibition at 1 mM, irreversible | Kitasatospora aureofaciens | |
| N-ethylmaleimide | complete inhibition at 0.1 M, irreversible | Kitasatospora aureofaciens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Kitasatospora aureofaciens | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | increase of activity | Kitasatospora aureofaciens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57500 | - |
2 * 57500, SDS-PAGE | Kitasatospora aureofaciens |
| 115000 | - |
gel filtration | Kitasatospora aureofaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anhydrotetracycline + NADPH + O2 | Kitasatospora aureofaciens | tetracycline and chlortetracycline biosynthetic pathways | 12-dehydrotetracycline + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kitasatospora aureofaciens | - |
50/137, UV-light induced mutant of strain 84/25 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Kitasatospora aureofaciens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, crude extract stable for several weeks | Kitasatospora aureofaciens |
| 4°C, crude extract stable for several days | Kitasatospora aureofaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anhydrotetracycline + NADPH + O2 | - |
Kitasatospora aureofaciens | 12-dehydrotetracycline + NADP+ + H2O | - |
? | |
| anhydrotetracycline + NADPH + O2 | tetracycline and chlortetracycline biosynthetic pathways | Kitasatospora aureofaciens | 12-dehydrotetracycline + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57500, SDS-PAGE | Kitasatospora aureofaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.4 | assay at | Kitasatospora aureofaciens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | increase of activity | Kitasatospora aureofaciens | |
| NADPH | - |
Kitasatospora aureofaciens | |
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