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Literature summary for 1.14.13.29 extracted from

  • Tassaneeyakul, W.; Veronese, M.E.; Birkett, D.J.; Gonzalez, F.J.; Miners, J.O.
    Validation of 4-nitrophenol as an in vitro substrate probe for human liver CYP2E1 using cDNA expression and microsomal kinetic techniques (1993), Biochem. Pharmacol., 46, 1975-1981.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
alpha-naphthoflavone 60% activiation at 25 mM Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenol mutual competitive inhibition with chlorzoxazone Homo sapiens
alpha-naphthoflavone slight Homo sapiens
chlorzoxazone mutual competitive inhibition with 4-nitrophenol Homo sapiens
coumarin slight Homo sapiens
diethyldithiocarbamate 50% inhibition at 0.002-0.003 mM Homo sapiens
mephenytoin slight Homo sapiens
quinidine slight Homo sapiens
sulfaphenazole slight Homo sapiens
troleandomycin slight Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
4-nitrocatechol cDNA expressed enzyme Homo sapiens
0.03
-
4-nitrocatechol
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
human
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenol + NADH + O2
-
Homo sapiens 4-nitrocatechol + NAD+ + H2O
-
?
chlorzoxazone + NADH + O2 6-hydroxylation Homo sapiens ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.042
-
4-nitrophenol inhibition of chlorzoxazone 6-hydroxylation Homo sapiens
0.047
-
chlorzoxazone inhibition of 4-nitrophenol hydroxylation Homo sapiens