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Literature summary for 1.14.13.29 extracted from

  • Mitra, D.; Vaidyanathan, C.S.
    A new 4-nitrophenol 2-hydroxylase from a Nocardia sp (1984), Biochem. Int., 8, 609-615.
    View publication on PubMed

General Stability

General Stability Organism
dialysis of crude enzyme extract, complete loss of activity Nocardia sp.

Inhibitors

Inhibitors Comment Organism Structure
catalase 30% inhibition at 1000 units Nocardia sp.
Co2+ slight effect, crude enzyme extract Nocardia sp.
Cu2+ 94% inhibition at 1 mM,crude enzyme extract Nocardia sp.
Fe2+ slight effect, crude enzyme extract Nocardia sp.
Fe3+ slight effect, crude enzyme extract Nocardia sp.
Hg2+ 63% inhibition at 1 mM, crude enzyme extract Nocardia sp.
Horseradish peroxidase complete inhibition at 25 units Nocardia sp.
additional information inhibition at high ionic strength of all common buffers and salts e.g. phosphate, Tris, KCl, (NH4)2SO4, 60% inhibition above 300 mM, crude enzyme extract Nocardia sp.
N-Methylmaleimide 84% inhibition at 5 mM Nocardia sp.
Ni2+ slight effect, crude enzyme extract Nocardia sp.
p-chloromercuribenzoate 81% inhibition at 1 mM Nocardia sp.
Sn2+ 57% inhibition at 1 mM,crude enzyme extract Nocardia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0083
-
4-nitrocatechol
-
Nocardia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Nocardia sp. 5829
-

Organism

Organism UniProt Comment Textmining
Nocardia sp.
-
-
-

Storage Stability

Storage Stability Organism
-20°C, 85% loss of activity after 10 days Nocardia sp.
0-4°C, 30% loss of activity after 7 days, 70% loss of activity after 14 days, crude enzyme extract Nocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-nitrophenol + NADH + O2
-
Nocardia sp. ?
-
?
4-nitrophenol + NADH + O2 NADPH: 50% of the activity with NADH Nocardia sp. 4-nitrocatechol + NAD+ + H2O
-
?
chlorzoxazone + NADH + O2
-
Nocardia sp. ?
-
?
additional information no substrates: 2-nitrophenol, 2,4-dinitrophenol Nocardia sp. ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
crude enzyme extract Nocardia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
crude enzyme extract Nocardia sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein, FMN cannot replace FAD Nocardia sp.
NADH
-
Nocardia sp.
NADPH 50% of the activity with NADH Nocardia sp.