General Stability | Organism |
---|---|
dialysis of crude enzyme extract, complete loss of activity | Nocardia sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
catalase | 30% inhibition at 1000 units | Nocardia sp. | |
Co2+ | slight effect, crude enzyme extract | Nocardia sp. | |
Cu2+ | 94% inhibition at 1 mM,crude enzyme extract | Nocardia sp. | |
Fe2+ | slight effect, crude enzyme extract | Nocardia sp. | |
Fe3+ | slight effect, crude enzyme extract | Nocardia sp. | |
Hg2+ | 63% inhibition at 1 mM, crude enzyme extract | Nocardia sp. | |
Horseradish peroxidase | complete inhibition at 25 units | Nocardia sp. | |
additional information | inhibition at high ionic strength of all common buffers and salts e.g. phosphate, Tris, KCl, (NH4)2SO4, 60% inhibition above 300 mM, crude enzyme extract | Nocardia sp. | |
N-Methylmaleimide | 84% inhibition at 5 mM | Nocardia sp. | |
Ni2+ | slight effect, crude enzyme extract | Nocardia sp. | |
p-chloromercuribenzoate | 81% inhibition at 1 mM | Nocardia sp. | |
Sn2+ | 57% inhibition at 1 mM,crude enzyme extract | Nocardia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
4-nitrocatechol | - |
Nocardia sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Nocardia sp. | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia sp. | - |
- |
- |
Storage Stability | Organism |
---|---|
-20°C, 85% loss of activity after 10 days | Nocardia sp. |
0-4°C, 30% loss of activity after 7 days, 70% loss of activity after 14 days, crude enzyme extract | Nocardia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-nitrophenol + NADH + O2 | - |
Nocardia sp. | ? | - |
? | |
4-nitrophenol + NADH + O2 | NADPH: 50% of the activity with NADH | Nocardia sp. | 4-nitrocatechol + NAD+ + H2O | - |
? | |
chlorzoxazone + NADH + O2 | - |
Nocardia sp. | ? | - |
? | |
additional information | no substrates: 2-nitrophenol, 2,4-dinitrophenol | Nocardia sp. | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
crude enzyme extract | Nocardia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
crude enzyme extract | Nocardia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, FMN cannot replace FAD | Nocardia sp. | |
NADH | - |
Nocardia sp. | |
NADPH | 50% of the activity with NADH | Nocardia sp. |