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Literature summary for 1.14.13.25 extracted from

  • Yu, Y.; Ramsay, J.A.; Ramsay, B.A.
    Use of allylthiourea to produce soluble methane monooxygenase in the presence of copper (2009), Appl. Microbiol. Biotechnol., 82, 333-339.
    View publication on PubMed
Search for more literature for 1.14.13.25

Application

Application Comment Organism
biotechnology method by which sMMO can be produced by strain OB3b while growing on methanol in copper-containing medium Methylosinus trichosporium OB3b

Inhibitors

Inhibitors Comment Organism Structure
Allylthiourea pMMO inhibitor and copper chelator. Without allylthiourea strain OB3b quickly loses sMMO activity whenever it is grown on NMS medium with methane as the sole carbon and energy source. No loss of sMMO activity when the growth substrate is switched from methane to methanol when allylthiourea is added to growth medium containing copper Methylosinus trichosporium OB3b
Cu2+ loss of sMMO activity with copper addition to a culture growing on methanol. Recovery of sMMO activity by addition of allylthiourea Methylosinus trichosporium OB3b

Organism

Organism UniProt Comment Textmining
Methylosinus trichosporium OB3b
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + NAD(P)H + O2
-
 Methylosinus trichosporium OB3b methanol + NAD(P)+ + H2O
-
 ?
naphthalene + NAD(P)H + O2
-
 Methylosinus trichosporium OB3b alpha-naphthol + beta-naphthol + NAD(P)+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
sMMO
-
 Methylosinus trichosporium OB3b
soluble methane monooxygenase
-
 Methylosinus trichosporium OB3b

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Methylosinus trichosporium OB3b
NADPH
-
 Methylosinus trichosporium OB3b