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Literature summary for 1.14.13.25 extracted from
- Role of the C-terminal region of the B component of Methylosinus trichosporium OB3b methane monooxygenase in the regulation of oxygen activation (2006), Biochemistry, 45, 1459-1469.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of enzyme component MMOB in Escherichia coli | Methylosinus trichosporium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of deletion mutants of subunit MMOB missing 5, 8, and 13 C-terminal residues, the mutations cause progressive decreases in the maximum steady-state turnover number, as well as lower apparent rate constants for formation of the key reaction cycle intermediate compound Q, the deletions result in substantial uncoupling at or before the P intermediate due to competition between slow H2O2 release from one of the intermediates and the reaction that carries this intermediate on to the next step in the cycle, which is slowed by the mutation | Methylosinus trichosporium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | effects of the C-terminal region of the B component MMOB and the active site diiron cluster of sMMO on the steady-state turnover, stopped-flow transient kinetics of the reaction cycle of wild-type and mutant enzymes, overview, deuterium kinetic isotope effect for the reaction of Q with methane, overview | Methylosinus trichosporium |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | active site diiron cluster | Methylosinus trichosporium |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methane + NAD(P)H + O2 | Methylosinus trichosporium | - |
methanol + NAD(P)+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylosinus trichosporium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme component MMOB from Escherichia coli by solubilization from the 12000 x g pellet with 8 M urea, followed by dilution for enzyme refolding, and gel filtration | Methylosinus trichosporium |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O | reaction cycle, via formation of the hydroperoxo intermediate compound P and the key reaction cycle intermediate compound Q, overview | Methylosinus trichosporium |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant enzyme component MMOB from Escherichia coli is solubilized from the 12000 x g pellet with 8 M urea, followed by dilution to 1 mg/ml protein for enzyme refolding | Methylosinus trichosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methane + NAD(P)H + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sMMO | - |
Methylosinus trichosporium |
| soluble methane monooxygenase | - |
Methylosinus trichosporium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Methylosinus trichosporium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.6 | assay at | Methylosinus trichosporium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Methylosinus trichosporium | |
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