Cloned (Comment) | Organism |
---|---|
expression of His-tagged triple mutant G10A/G13Q/G16A and G13Q mutant in Escherichia coli | Methylococcus capsulatus |
Protein Variants | Comment | Organism |
---|---|---|
G10A/G13Q/G16A | reduced activity | Methylococcus capsulatus |
G10A/G13Q/G16A | His-tagged protein B of sMMO, triple mutant is resistant to degradation in contrast to the wild-type, N-terminus is responsible for unusual mobility in size exclusion chromatography and proteolytic sensitivity of protein B | Methylococcus capsulatus |
G13Q | reduced activity | Methylococcus capsulatus |
G13Q | sMMO, alteration of a cleavage site in component protein B | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
protein B shows unusual behaviour in gel filtration | Methylococcus capsulatus |
15850 | - |
component protein B of sMMO, mass spectrometry | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
Bath | - |
Methylococcus capsulatus | - |
enzyme form sMMO | - |
Methylococcus capsulatus Bath | - |
Bath | - |
Purification (Comment) | Organism |
---|---|
sMMO protein B wild-type and mutants recombinant from Escherichia coli by affinity chromatography, high salt concentration increases the binding stability between protein B and hydroxylase of sMMO | Methylococcus capsulatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O | structural model for component protein B of sMMO | Methylococcus capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.66 | - |
sMMO protein B triple mutant G10A/G13Q/G16A | Methylococcus capsulatus |
2.284 | - |
His-tagged sMMO protein B triple mutant G10A/G13Q/G16A | Methylococcus capsulatus |
3.99 | - |
sMMO protein B mutant G13Q | Methylococcus capsulatus |
5.09 | - |
sMMO, wild-type enzyme | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NAD(P)H + O2 | - |
Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
methane + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | methanol + NAD(P)+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MMO | - |
Methylococcus capsulatus |
pMMO | particulate, membrane-bound enzyme form | Methylococcus capsulatus |
sMMO | soluble, cytoplasmic enzyme form | Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylococcus capsulatus |