Literature summary for 1.14.13.25 extracted from

Lloyd, J.S.; Bhambra, A.; Murrell, J.C.; Dalton, H.
Inactivation of the regulatory protein B of soluble methane monooxygenase from Methylococcus capsulatus (Bath) by proteolysis can be overcome by a Gly to Gln modification (1997), Eur. J. Biochem., 248, 72-79.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of component protein B of sMMO as glutathione-S-transferase fusion protein in Escherichia coli	Methylococcus capsulatus

Protein Variants

Protein Variants	Comment	Organism
G13Q	enhanced temperature stability compared to wild-type, site-directed mutagenesis	Methylococcus capsulatus
G13Q	sMMO, alteration of a cleavage site in component protein B	Methylococcus capsulatus
additional information	native parallel occurence of full length and 2 N-terminal truncated forms of regulatory component protein B of sMMO, truncated forms are inactive	Methylococcus capsulatus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	cytoplasmatic, soluble enzyme form termed sMMO	Methylococcus capsulatus	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
15850	-	component protein B of sMMO, mass spectrometry	Methylococcus capsulatus

Organism

Organism	UniProt	Comment	Textmining
Methylococcus capsulatus	-	enzyme form sMMO	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant component protein B of sMMO as glutathione-S-transferase fusion protein from Escherichia coli	Methylococcus capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	recombinant shows higher activity than the wild-type	Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methane + NAD(P)H + O2	-	Methylococcus capsulatus	methanol + NAD(P)+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
MMO	-	Methylococcus capsulatus
pMMO	particulate, membrane-bound enzyme form	Methylococcus capsulatus
sMMO	soluble, cytoplasmic enzyme form	Methylococcus capsulatus

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Methylococcus capsulatus

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Release 2023.1 (January 2023)