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Literature summary for 1.14.13.25 extracted from

  • Woodland, M.P.; Dalton, H.
    Purification and characterization of component A of the methane monooxygenase from Methylococcus capsulatus (Bath) (1984), J. Biol. Chem., 259, 53-59.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 2.3 mol Fe per mol protein Methylococcus capsulatus
Zn2+ 0.2-0.5 mol zinc per mol protein Methylococcus capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
see under subunits: molecular weights of the subunits of components Methylococcus capsulatus
17000
-
component A: 2 * 54000 alpha + 2 * 42000 beta + 2 * 17000 gamma, SDS-PAGE and analytical ultracentrifugation Methylococcus capsulatus
42000
-
component A: 2 * 54000 alpha + 2 * 42000 beta + 2 * 17000 gamma, SDS-PAGE and analytical ultracentrifugation Methylococcus capsulatus
54000
-
component A: 2 * 54000 alpha + 2 * 42000 beta + 2 * 17000 gamma, SDS-PAGE and analytical ultracentrifugation Methylococcus capsulatus
210000
-
component A, analytical ultracentrifugation Methylococcus capsulatus

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
Bath
-
Methylococcus capsulatus Bath
-
Bath
-

Purification (Commentary)

Purification (Comment) Organism
component A Methylococcus capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.072
-
component A Methylococcus capsulatus

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, component A, stable for several weeks Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + NAD(P)H + O2
-
Methylococcus capsulatus methanol + NAD(P)+ + H2O
-
?
methane + NAD(P)H + O2
-
Methylococcus capsulatus Bath methanol + NAD(P)+ + H2O
-
?

Subunits

Subunits Comment Organism
? component A: 2 * 54000 alpha + 2 * 42000 beta + 2 * 17000 gamma, SDS-PAGE and analytical ultracentrifugation Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methylococcus capsulatus