Literature summary for 1.14.13.25 extracted from

Patel, R.N.; Savas, J.C.
Purification and properties of the hydroxylase component of methane monooxygenase (1987), J. Bacteriol., 169, 2313-2317.

Search for more literature for 1.14.13.25

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	2.8 mol Fe per mol protein	Methylobacterium sp.
Zn2+	component A, hydroxylase component: contains 0.5 mol zinc per mol protein	Methylobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	see under subunits: molecular weights of the subunits of components	Methylobacterium sp.
additional information	-	enzyme system consists of 3 protein components A, B, C	Methylobacterium sp.
20000	-	component A: 2 * 55000 alpha + 2 * 40000 beta + 2 * 20000 gamma, SDS-PAGE	Methylobacterium sp.
40000	-	component A: 2 * 55000 alpha + 2 * 40000 beta + 2 * 20000 gamma, SDS-PAGE	Methylobacterium sp.
55000	-	component A: 2 * 55000 alpha + 2 * 40000 beta + 2 * 20000 gamma, SDS-PAGE	Methylobacterium sp.
220000	-	component A: hydroxylase, gel filtration	Methylobacterium sp.

Organism

Organism	UniProt	Comment	Textmining
Methylobacterium sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
hydroxylase component	Methylobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.208	-	purified component A hydroxylase	Methylobacterium sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methane + NAD(P)H + O2	-	Methylobacterium sp.	methanol + NAD(P)+ + H2O	-	?

Subunits

Subunits	Comment	Organism
?	component A: 2 * 55000 alpha + 2 * 40000 beta + 2 * 20000 gamma, SDS-PAGE	Methylobacterium sp.
More	see under molecular weight for the size of the protein components	Methylobacterium sp.
More	enzyme system consists of 3 protein components A, B, C	Methylobacterium sp.

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Methylobacterium sp.

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Release 2023.1 (January 2023)