Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Rhodococcus jostii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000 | Rhodococcus jostii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADH + H+ + O2 | Rhodococcus jostii | - |
2,5-dihydroxybenzoate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography and Sephadex G-25 gel filtration | Rhodococcus jostii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADH + H+ + O2 | - |
Rhodococcus jostii | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 47000 | Rhodococcus jostii |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxybenzoate 6-hydroxylase | - |
Rhodococcus jostii |
3HB6H | - |
Rhodococcus jostii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains one FAD per subunit | Rhodococcus jostii | |
NADH | - |
Rhodococcus jostii |