Cloned (Comment) | Organism |
---|---|
gene mhbM, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha | Klebsiella oxytoca |
Protein Variants | Comment | Organism |
---|---|---|
R169E | site-directed mutagenesis, inactive mutant | Klebsiella oxytoca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, about, SDS-PAGE | Klebsiella oxytoca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADH + O2 | Klebsiella oxytoca | - |
2,5-dihydroxybenzoate + NAD+ + H2O | i.e. gentisate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella oxytoca | Q5EXK1 | gene mhbM | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-hydroxybenzoate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O | Arg169 is essential for catalytic activity | Klebsiella oxytoca |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.53 | - |
recombinant wild-type enzyme | Klebsiella oxytoca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADH + O2 | - |
Klebsiella oxytoca | 2,5-dihydroxybenzoate + NAD+ + H2O | i.e. gentisate | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, about, SDS-PAGE | Klebsiella oxytoca |
More | Ser166 and Arg169 form the highly conserved SXXR motif important for structure and function of the enzyme | Klebsiella oxytoca |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxybenzoate 6-hydroxylase | - |
Klebsiella oxytoca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Klebsiella oxytoca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Klebsiella oxytoca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Klebsiella oxytoca | |
NADH | - |
Klebsiella oxytoca |