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Literature summary for 1.14.13.24 extracted from

  • Groseclose, E.E.; Ribbons, D.W.
    3-Hydroxybenzoate 6-hydroxylase from Pseudomonas aeruginosa (1973), Biochem. Biophys. Res. Commun., 55, 897-903.
    View publication on PubMed
Search for more literature for 1.14.13.24

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 NADH
-
 Pseudomonas aeruginosa
0.8
-
 NADPH
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 sedimentation equilibrium measurement Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa T1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybenzoate + NADH + O2 10.5% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa 2,3,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
2,3-dihydroxybenzoate + NADH + O2 10.5% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa T1 2,3,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
3,4-dihydroxybenzoate + NADH + O2 9% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa 2,4,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
3,4-dihydroxybenzoate + NADH + O2 9% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa T1 2,4,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
3,5-dihydroxybenzoate + NADH + O2 7% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa 2,3,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
3,5-dihydroxybenzoate + NADH + O2 7% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa T1 2,3,5-trihydroxybenzoate + NAD+ + H2O
-
 ?
3-hydroxy-5-methylbenzoate + NADH + O2 31% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa 2,5-dihydroxy-3-methylbenzoate + NAD+ + H2O
-
 ?
3-hydroxy-5-methylbenzoate + NADH + O2 31% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa T1 2,5-dihydroxy-3-methylbenzoate + NAD+ + H2O
-
 ?
3-hydroxybenzoate + NADH + O2
-
 Pseudomonas aeruginosa 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?
3-hydroxybenzoate + NADH + O2
-
 Pseudomonas aeruginosa T1 2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?
3-hydroxybenzoate + NADPH + H+ + O2
-
 Pseudomonas aeruginosa 2,5-dihydroxybenzoate + NADP+ + H2O
-
 r
4-fluoro-3-hydroxybenzoate + NADH + O2 36% of the reaction with 3-hydroxybenzoate Pseudomonas aeruginosa 4-fluoro-2,5-dihydroxybenzoate + NAD+ + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Pseudomonas aeruginosa
FAD 0.9 mol of FAD per mol of enzyme Pseudomonas aeruginosa
NADH
-
 Pseudomonas aeruginosa
NADPH can act instead of NADH, more slowly Pseudomonas aeruginosa