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Literature summary for 1.14.13.22 extracted from

  • Secundo, F.; Zambianchi, F.; Crippa, G.; Carrea, G.; Tedeschi, G.
    Comparative study of the properties of wild type and recombinant cyclohexanone monooxygenase, an enzyme of synthetic interest (2005), J. Mol. Catal. B, 34, 1-6.
No PubMed abstract available
Search for more literature for 1.14.13.22

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is inducible by cyclohexanone Acinetobacter calcoaceticus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli under control of a strong L-arabinose inducible promoter Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 bicyclo[3.2.0]hept-2-en-6-one pH 8.6, 25°C, recombinant and native enzyme Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclohexanone + NADPH + O2 Acinetobacter calcoaceticus
-
 hexano-6-lactone + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
 an inducible enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 21fold from Escherichia coli and native enzyme to homogeneity by ion exchange and affinity chromatography Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.1
-
 purified recombinant enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one Acinetobacter calcoaceticus
9.2
-
 purified native enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bicyclo[3.2.0]hept-2-en-6-one + NADPH + O2
-
 Acinetobacter calcoaceticus ?
-
 ?
cyclohexanone + NADPH + O2
-
 Acinetobacter calcoaceticus hexano-6-lactone + NADP+ + H2O
-
 ?
additional information the enzyme catalyses the NADPH-dependent enantioselective oxidation of ketones and of several heteroatoms such as nitrogen, sulfur, phosphorous and selenium present in organic compounds Acinetobacter calcoaceticus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61586.04, recombinant enzyme, mass spectrometry, x * 61583.54, native enzyme, mass spectrometry Acinetobacter calcoaceticus

Synonyms

Synonyms Comment Organism
CHMO
-
 Acinetobacter calcoaceticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 assay at Acinetobacter calcoaceticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 comparison of pH stability of crude, partially purified, and purified native and recombinant enzymes, overview Acinetobacter calcoaceticus
6
-
 48 h, purified recombinant enzyme: loss of 12% activity, native enzyme: loss of 24% activity Acinetobacter calcoaceticus
8.6
-
 48 h, purified recombinant enzyme: loss of 15% activity, native enzyme: loss of 24% activity Acinetobacter calcoaceticus
10
-
 48 h, purified recombinant enzyme: loss of 45% activity, native enzyme: loss of 82% activity Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
FAD flavoenzyme Acinetobacter calcoaceticus
NADPH dependent on Acinetobacter calcoaceticus