Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | completely | Exophiala jeanselmei | |
Cu2+ | completely | Exophiala jeanselmei | |
Quinacrine | - |
Exophiala jeanselmei | |
Quinine | - |
Exophiala jeanselmei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00048 | - |
cyclohexanone | - |
Exophiala jeanselmei | |
0.0142 | - |
NADPH | - |
Exophiala jeanselmei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
gel filtration | Exophiala jeanselmei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Exophiala jeanselmei | - |
- |
- |
Exophiala jeanselmei | - |
inducible by cyclohexanone | - |
Exophiala jeanselmei KUFI-6N | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Exophiala jeanselmei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
678 | - |
purified enzyme | Exophiala jeanselmei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclobutanone + NADPH + O2 | - |
Exophiala jeanselmei | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
cyclobutanone + NADPH + O2 | - |
Exophiala jeanselmei KUFI-6N | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
cyclodecanone + NADPH + O2 | low activity | Exophiala jeanselmei | 1-oxa-2-oxocycloendecane + NADP+ + H2O | - |
? | |
cycloheptanone + NADPH + O2 | - |
Exophiala jeanselmei | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
cycloheptanone + NADPH + O2 | - |
Exophiala jeanselmei KUFI-6N | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | - |
Exophiala jeanselmei | 6-hexanolide + NADP+ + H2O | product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane | ? | |
cyclohexanone + NADPH + O2 | - |
Exophiala jeanselmei KUFI-6N | 6-hexanolide + NADP+ + H2O | product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane | ? | |
cyclooctanone + NADPH + O2 | - |
Exophiala jeanselmei | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
cyclooctanone + NADPH + O2 | - |
Exophiala jeanselmei KUFI-6N | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
cyclopentanone + NADPH + O2 | - |
Exophiala jeanselmei | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
cyclopentanone + NADPH + O2 | - |
Exophiala jeanselmei KUFI-6N | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 74000, SDS-PAGE | Exophiala jeanselmei |
Synonyms | Comment | Organism |
---|---|---|
CHMO | - |
Exophiala jeanselmei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Exophiala jeanselmei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Exophiala jeanselmei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | no activity below pH 5.5 and above pH 10.5 at 30°C | Exophiala jeanselmei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | stable | Exophiala jeanselmei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | binding motif, comparison of N-terminal amino acid sequences of several species | Exophiala jeanselmei | |
NADPH | - |
Exophiala jeanselmei |