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Literature summary for 1.14.13.22 extracted from

  • Norris, D.B.; Trudgill, P.W.
    Multiple forms of cyclohexanone oxygenase from Nocardia globerula CL1 (1976), Eur. J. Biochem., 63, 193-198.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Nocardia globerula
0.0185
-
NADPH isozyme type 1 Nocardia globerula
0.02
-
NADPH isozyme type 2 Nocardia globerula

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Nocardia globerula 5737
-

Organism

Organism UniProt Comment Textmining
Nocardia globerula
-
enzyme exists in 2 electrophoretically distinct isoforms: type 1 and type 2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxycyclohexanone + NADPH + O2
-
Nocardia globerula 1-oxa-2-oxo-3-hydroxycycloheptane + NADP+ + H2O
-
?
2-methylcyclohexanone + NADPH + O2
-
Nocardia globerula 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O
-
?
cyclobutanone + NADPH + O2
-
Nocardia globerula 1-oxa-2-oxo-cyclopentane + NADP+ + H2O
-
?
cycloheptanone + NADPH + O2
-
Nocardia globerula 1-oxa-2-oxo-cyclooctane + NADP+ + H2O
-
?
cyclohexanone + NADPH + O2
-
Nocardia globerula 6-hexanolide + NADP+ + H2O
-
?
cyclooctanone + NADPH + O2
-
Nocardia globerula 1-oxa-2-oxo-cyclononane + NADP+ + H2O
-
?
cyclopentanone + NADPH + O2
-
Nocardia globerula 1-oxa-2-oxo-cyclohexane + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 58000-59000, SDS-PAGE Nocardia globerula

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
2 min, 40% loss of activity for isozyme type 1, 7% loss of activity for isozyme type 2 Nocardia globerula
45
-
1 min, 40% loss of activity for isozyme type 1, 18% loss of activity for isozyme type 2 Nocardia globerula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 10.1 broad, isozyme type 2 Nocardia globerula
8
-
sharp, isozyme type 1 Nocardia globerula

pH Range

pH Minimum pH Maximum Comment Organism
6 10.5 pH 6.0: about 40% of activity maximum with isozymes type 1 and type 2, pH 10.5: about 10% of activity maximum with isozyme type 1, about 60% of activity maximum with isozyme type 2 Nocardia globerula

Cofactor

Cofactor Comment Organism Structure
FAD 0.62 mol of FAD per mol of holoenzyme type 1, 0.75 mol of FAD per mol of holoenzyme type 2 Nocardia globerula
NADPH absolutely specific for NADPH as electron donor Nocardia globerula