Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pseudomonas sp. | 5737 | - |
cytoplasm | - |
Xanthobacter sp. | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
1 * 51000, SDS-PAGE | Xanthobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Xanthobacter sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Xanthobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
- |
Xanthobacter sp. |
Storage Stability | Organism |
---|---|
-20°C, stable | Xanthobacter sp. |
4°C, in presence of cyclohexane or 0.1 mM NADPH | Xanthobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phenyl-2-propanone + NADPH + O2 | - |
Pseudomonas sp. | ? | - |
? | |
1-phenyl-2-propanone + NADPH + O2 | - |
Xanthobacter sp. | ? | - |
? | |
2-phenyl-1-ethanal + NADPH + O2 | - |
Pseudomonas sp. | ? | - |
? | |
2-phenyl-1-ethanal + NADPH + O2 | - |
Xanthobacter sp. | ? | - |
? | |
cyclohexanone + NADPH + O2 | - |
Xanthobacter sp. | 6-hexanolide + NADP+ + H2O | - |
? | |
additional information | no activity towards straight-chain alkanones | Pseudomonas sp. | ? | - |
? | |
additional information | no activity towards straight-chain alkanones | Xanthobacter sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 51000, SDS-PAGE | Xanthobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas sp. |
30 | - |
assay at | Xanthobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Xanthobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | 1 FMN molecule per molecule of protein | Xanthobacter sp. | |
NADPH | absolutely specific for NADPH as electron donor | Pseudomonas sp. | |
NADPH | absolutely specific for NADPH as electron donor | Xanthobacter sp. |