Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Thiacyclohexanone | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | |
2-Thiocyclohexanone | - |
Acinetobacter sp. | |
delta-thiovalerolactone | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | |
ethylene monothiocarbonate | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | |
additional information | inactivation mechanism | Acinetobacter sp. | |
S-gamma-Thiobutyrolactone | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
cyclohexanone | - |
Acinetobacter sp. | |
0.01 | - |
3-thiacyclohexanone | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | |
0.016 | - |
4-methylcyclohexanone | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | |
0.021 | - |
4-thiacyclohexanone | - |
Acinetobacter sp. | |
0.024 | - |
Thiane | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
NCIB 9871 | - |
Purification (Comment) | Organism |
---|---|
partially | Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8 | 10 | partially purified enzyme, substrate 4-methylcyclohexanone | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-thiacyclohexanone + NADPH + O2 | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | 1-oxa-2-oxo-3-thiacycloheptane + NADP+ + H2O | - |
? | |
3-thiacyclohexanone + NADPH + H+ + O2 | - |
Acinetobacter sp. | ? | - |
r | |
4-methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-5-methylcycloheptane + NADP+ + H2O | - |
? | |
4-thiacyclohexanone + NADPH + H+ + O2 | - |
Acinetobacter sp. | ? | - |
r | |
cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 6-hexanolide + NADP+ + H2O | - |
? | |
delta-thiovalerolactone + NADPH + O2 | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | ? | - |
ir | |
epsilon-thiocaprolactone + NADPH + O2 | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | ? | - |
ir | |
ethylene monothiocarbonate + NADPH + O2 | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | ? | - |
? | |
ethylene monothiocarbonate + NADPH + O2 | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | ? | - |
ir | |
gamma-thiobutyrolactone + NADPH + O2 | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | ? | - |
ir | |
S-gamma-thiobutyrolactone + NADPH + O2 | substrate irreversibly inactivates enzyme after a few turnovers | Acinetobacter sp. | ? | - |
? | |
thiane + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.227 | - |
epsilon-thiocaprolactone | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | |
0.24 | - |
delta-thiovalerolactone | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | |
0.683 | - |
gamma-thiobutyrolactone | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. | |
2.38 | - |
ethylene monothiocarbonate | substrate inactivates enzyme after a few turnovers | Acinetobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Acinetobacter sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
ethylene monothiocarbonate | - |
Acinetobacter sp. | |
2 | - |
S-gamma-Thiobutyrolactone | - |
Acinetobacter sp. | |
2 | - |
2-Thiocyclohexanone | - |
Acinetobacter sp. | |
2.9 | - |
delta-thiovalerolactone | - |
Acinetobacter sp. |