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Literature summary for 1.14.13.22 extracted from

  • Trower, M.K.; Buckland, R.M.; Griffin, M.
    Characterization of an FMN-containing cyclohexanone monooxygenase from a cyclohexane-grown Xanthobacter sp (1989), Eur. J. Biochem., 181, 199-206.
    View publication on PubMed
Search for more literature for 1.14.13.22

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Xanthobacter sp.
arsenate
-
 Xanthobacter sp.
CuCl2
-
 Xanthobacter sp.
FeCl3
-
 Xanthobacter sp.
p-hydroxymercuribenzoate
-
 Xanthobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 cyclohexanone below Xanthobacter sp.
0.0125
-
 NADPH
-
 Xanthobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Xanthobacter sp. 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Xanthobacter sp.
51000
-
 1 * 51000, SDS-PAGE Xanthobacter sp.

Organism

Organism UniProt Comment Textmining
Xanthobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xanthobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
-
 Xanthobacter sp.

Storage Stability

Storage Stability Organism
-20°C, stable Xanthobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxycyclohexanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxo-3-hydroxycycloheptane + NADP+ + H2O
-
 ?
2-methylcyclohexanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O
-
 ?
cyclobutanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxo-cyclopentane + NADP+ + H2O
-
 ?
cycloheptanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxo-cyclooctane + NADP+ + H2O
-
 ?
cyclohexan-1,2-dione + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2,3-dioxo-cycloheptane + NADP+ + H2O
-
 ?
cyclohexan-1,4-dione + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2,5-dioxo-cycloheptane + NADP+ + H2O
-
 ?
cyclohexanone + NADPH + O2
-
 Xanthobacter sp. 6-hexanolide + NADP+ + H2O
-
 ?
cyclooctanone + NADPH + O2 low activity Xanthobacter sp. 1-oxa-2-oxo-cyclononane + NADP+ + H2O
-
 ?
cyclopentanone + NADPH + O2
-
 Xanthobacter sp. 1-oxa-2-oxo-cyclohexane + NADP+ + H2O
-
 ?
additional information no activity towards straight-chain alkanones Xanthobacter sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 51000, SDS-PAGE Xanthobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Xanthobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Xanthobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 11 at pH 6.0 and 11.0: about 10% of activity maximum Xanthobacter sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Xanthobacter sp.
FMN involved in catalytic mechanism Xanthobacter sp.
FMN 1 FMN molecule per molecule of protein Xanthobacter sp.
NADPH electron donor Xanthobacter sp.