Activating Compound | Comment | Organism | Structure |
---|---|---|---|
protein AsuE2 | endogenous protein AsuE2, UniProt-ID D7P5W0, is necessary for the full catalytic function of AsuE1 as a two-component flavoprotein hydroxylase. AsuE2 is a homologue of the flavin reductase PheA2, which recycles oxidized FADox and provides FADred to its hydroxylase partner PheA1 in the phenol hydroxylation in Bacillus thermoglucosidasius A7 | Streptomyces nodosus |
Cloned (Comment) | Organism |
---|---|
cloning of asukamycin biosynthetic gene cluster, including gene asuE1 encoding the enzyme, recombinant expression in Escherichia coli strain BW25113/pIJ790, heterologous expression of the complete gene cluster in the asukamycin-nonproducing Streptomyces lividans strain K4-114 | Streptomyces nodosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoasukamycin + NADH + H+ + O2 | Streptomyces nodosus | - |
4-hydroxyprotoasukamycin + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces nodosus | D7P5V0 | subsp. asukaensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoasukamycin + NADH + H+ + O2 | - |
Streptomyces nodosus | 4-hydroxyprotoasukamycin + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
asuE1 | - |
Streptomyces nodosus |
protoasukamycin 4-hydroxylase | - |
Streptomyces nodosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD is recycled by enzyme AsuE2 | Streptomyces nodosus | |
NADH | - |
Streptomyces nodosus |
General Information | Comment | Organism |
---|---|---|
metabolism | in the asukamycin metabolism, enzymes AsuE1 and AsuE2 catalyze the conversion of protoasukamycin to 4-hydroxyprotoasukamycin, which is epoxidized at C5-C6 by enzyme AsuE3 to the final product, asukamycin. Synthesis and structures of asukamycin and related metabolites, overview | Streptomyces nodosus |