Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of tfdBI and tfdBII inactivation mutants on pJP4 plasmid, constructed in Escherichia coli strain BW25113 by allelic replacement of each of the genes tfdBI or tfdBII with a kanamycin resistance cassette, tfdBI and tfdBII are required for growth on 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, with tfdBI contributing to a higher extent than tfdBII, phenotype, overview, accumulation of chlorophenol inhibits chlorophenoxyacetate degradation, and inactivation of the tfdB genes enhances the toxic effect of 2,4-dichlorophenoxyacetate on Cupriavidus necator cells | Cupriavidus necator |
additional information | overexpression of tdfA inhibits chlorophenoxyacetate degradation in Burkholderia xenovorans/pJP4 cells | Paraburkholderia xenovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenol + NADPH + O2 | Cupriavidus necator | - |
3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
2,4-dichlorophenol + NADPH + O2 | Paraburkholderia xenovorans | - |
3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
4-chloro-2-methylphenol + NADPH + O2 | Cupriavidus necator | - |
? | - |
? | |
4-chloro-2-methylphenol + NADPH + O2 | Paraburkholderia xenovorans | - |
? | - |
? | |
4-chlorophenol + NADPH + O2 | Cupriavidus necator | - |
5-chlorocatechol + NADP+ + H2O | - |
? | |
4-chlorophenol + NADPH + O2 | Paraburkholderia xenovorans | - |
5-chlorocatechol + NADP+ + H2O | - |
? | |
additional information | Cupriavidus necator | the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview | ? | - |
? | |
additional information | Paraburkholderia xenovorans | the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
strains JMP222 and JMP134 containing the enzyme-encoding plasmid pJP4, genes tfdBI and tfdBII | - |
Paraburkholderia xenovorans | - |
containing the enzyme-encoding plasmid pJP4, genes tfdBI and tfdBII | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
in vivo degradation activities, overview | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenol + NADPH + O2 | - |
Cupriavidus necator | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
2,4-dichlorophenol + NADPH + O2 | - |
Paraburkholderia xenovorans | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
4-chloro-2-methylphenol + NADPH + O2 | - |
Cupriavidus necator | ? | - |
? | |
4-chloro-2-methylphenol + NADPH + O2 | - |
Paraburkholderia xenovorans | ? | - |
? | |
4-chlorophenol + NADPH + O2 | - |
Cupriavidus necator | 5-chlorocatechol + NADP+ + H2O | - |
? | |
4-chlorophenol + NADPH + O2 | - |
Paraburkholderia xenovorans | 5-chlorocatechol + NADP+ + H2O | - |
? | |
additional information | the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview | Cupriavidus necator | ? | - |
? | |
additional information | the enzyme catabolizes 2,4-dichlorophenoxyacetate and 4-chloro-2-methylphenoxyacetate, using tfd functions carried on plasmid pJP4, tfdA cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol and 4-chloro-2-methylphenol, respectively, these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdBI and tfdBII genes to produce the respective chlorocatechols, degradation pathway, a balance between chlorophenol-producing and chlorophenol-consuming reactions is necessary for growth on these compounds, overview | Paraburkholderia xenovorans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chlorophenol hydroxylase | - |
Cupriavidus necator |
chlorophenol hydroxylase | - |
Paraburkholderia xenovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cupriavidus necator |
30 | - |
assay at | Paraburkholderia xenovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Cupriavidus necator |
7.6 | - |
assay at | Paraburkholderia xenovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Cupriavidus necator | |
NADPH | - |
Paraburkholderia xenovorans |