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Literature summary for 1.14.13.20 extracted from

  • Liu, T.; Chapman, P.J.
    Purification and properties of a plasmid-encoded 2,4-dichlorophenol hydroxylase (1984), FEBS Lett., 173, 314-318.
    View publication on PubMed
Search for more literature for 1.14.13.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pseudomonas putida Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
2,4-Dichlorophenol above 1 mM Cupriavidus necator
AgNO3
-
 Cupriavidus necator
CuSO4
-
 Cupriavidus necator
diethyldithiocarbamate
-
 Cupriavidus necator
Dithionitrobenzoate
-
 Cupriavidus necator
dithiothreitol slight Cupriavidus necator
FeSO4
-
 Cupriavidus necator
Hg2+ HgCl2 Cupriavidus necator
KCl high salt concentration inhibits, 0.05 M: 40%, 0.1 M: 50%, 0.2 M: 70% Cupriavidus necator
KCN no effect Cupriavidus necator
o-phenanthroline
-
 Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 2,4-Dichlorophenol cosubstrate NADPH Cupriavidus necator
0.004
-
 2,4-Dichlorophenol cosubstrate NADH Cupriavidus necator
0.048
-
 NADPH + 2,4-dichlorophenol Cupriavidus necator
0.3
-
 NADH + 2,4-dichlorophenol Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE Cupriavidus necator
67000
-
 alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE Cupriavidus necator
224000
-
 ultracentrifugation Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dichlorophenol + NADPH + O2 Cupriavidus necator
-
 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
2,4-dichlorophenol + NADPH + O2 Cupriavidus necator JMP 134-1
-
 3,5-dichlorocatechol + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
 basonym Alcaligenes eutrophus
-
Cupriavidus necator
-
 JMP134
-
Cupriavidus necator JMP 134-1
-
 JMP134
-

Purification (Commentary)

Purification (Comment) Organism
plasmid pJP4-encoded enzyme from Alcaligenes eutrophus JMP134 transferred to Pseudomonas putida 3CB5 and Alcaligenes eutrophus 335 Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.48
-
-
 Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4,5-trichlorophenol + NADPH + O2
-
 Cupriavidus necator ?
-
 ?
2,4,5-trichlorophenol + NADPH + O2
-
 Cupriavidus necator JMP 134-1 ?
-
 ?
2,4-dibromophenol + NADPH + O2
-
 Cupriavidus necator ?
-
 ?
2,4-dibromophenol + NADPH + O2
-
 Cupriavidus necator JMP 134-1 ?
-
 ?
2,4-dichlorophenol + NADH + H+ + O2
-
 Cupriavidus necator 3,5-dichlorocatechol + NAD+ + H2O
-
 r
2,4-dichlorophenol + NADPH + O2
-
 Cupriavidus necator 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
2,4-dichlorophenol + NADPH + O2
-
 Cupriavidus necator JMP 134-1 3,5-dichlorocatechol + NADP+ + H2O
-
 ?
2-chloro-4-nitrophenol + NADPH + O2
-
 Cupriavidus necator ?
-
 ?
2-chlorophenol + NADPH + O2 incompletely metabolized Cupriavidus necator ?
-
 ?
2-chlorophenol + NADPH + O2 incompletely metabolized Cupriavidus necator JMP 134-1 ?
-
 ?
4-chloro-2-methylphenol + NADPH + O2
-
 Cupriavidus necator ?
-
 ?
4-chloro-2-methylphenol + NADPH + O2
-
 Cupriavidus necator JMP 134-1 ?
-
 ?
4-chloro-2-nitrophenol + NADPH + O2
-
 Cupriavidus necator ?
-
 ?
4-chlorophenol + NADPH + O2
-
 Cupriavidus necator ?
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha2beta2, 2 * 67000 + 2 * 45000, SDS-PAGE Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 8 phosphate buffer Cupriavidus necator
8.1 8.5 100 mM Tris-HCl buffer Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Cupriavidus necator
FAD 1 mol of enzyme contains: 3.5 mol FAD Cupriavidus necator
NADH 50% of the activity with NADPH Cupriavidus necator
NADPH
-
 Cupriavidus necator