Cloned (Comment) | Organism |
---|---|
expression of wild-type and E49Q mutant PHPH in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E49Q | mutant has lost the ability in the oxidized state to rapidly exchange the product, i.e., 3,4-dihydroxybenzoate, for the substrate, p-hydroxybenzoate | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | competitive inhibition | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | Pseudomonas aeruginosa | - |
protocatechuate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PHBH | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas aeruginosa | protocatechuate + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PHBH | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
p-hydroxybenzoate | 4°C, pH 6.5, E49Q mutant | Pseudomonas aeruginosa | |
1.5 | - |
p-hydroxybenzoate | 4°C, pH 8.6, E49Q mutant | Pseudomonas aeruginosa | |
6.2 | - |
p-hydroxybenzoate | 4°C, pH 6.5, wild-type PHBH | Pseudomonas aeruginosa | |
7.5 | - |
p-hydroxybenzoate | 4°C, pH 8.6, wild-type PHBH | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas aeruginosa | |
NADPH | - |
Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
phosphate | 4°C, pH 6.8 | Pseudomonas aeruginosa |