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Literature summary for 1.14.13.2 extracted from

  • Ortiz-Maldonado, M.; Cole, L.J.; Dumas, S.M.; Entsch, B.; Ballou, D.P.
    Increased positive electrostatic potential in p-hydroxybenzoate hydroxylase accelerates hydroxylation but slows turnover (2004), Biochemistry, 43, 1569-1579.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and E49Q mutant PHPH in Escherichia coli Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
E49Q mutant has lost the ability in the oxidized state to rapidly exchange the product, i.e., 3,4-dihydroxybenzoate, for the substrate, p-hydroxybenzoate Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
phosphate competitive inhibition Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxybenzoate + NADPH + O2 Pseudomonas aeruginosa
-
protocatechuate + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PHBH Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzoate + NADPH + O2
-
Pseudomonas aeruginosa protocatechuate + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
PHBH
-
Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
p-hydroxybenzoate 4°C, pH 6.5, E49Q mutant Pseudomonas aeruginosa
1.5
-
p-hydroxybenzoate 4°C, pH 8.6, E49Q mutant Pseudomonas aeruginosa
6.2
-
p-hydroxybenzoate 4°C, pH 6.5, wild-type PHBH Pseudomonas aeruginosa
7.5
-
p-hydroxybenzoate 4°C, pH 8.6, wild-type PHBH Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas aeruginosa
NADPH
-
Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3
-
phosphate 4°C, pH 6.8 Pseudomonas aeruginosa