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Literature summary for 1.14.13.2 extracted from

  • Seibold, B.; Matthes, M.; Eppink, M.H.M.; Lingens, F.; Van Berkel, W.J.H.; Mueller, R.
    4-Hydroxybenzoate hydroxylase from Pseudomonas sp. CBS3. Purification, characterization, gene cloning, sequence analysis and assignment of structural features determining the coenzyme specificity (1996), Eur. J. Biochem., 239, 469-478.
    View publication on PubMed
Search for more literature for 1.14.13.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cl- competitive with respect to NADH Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 4-hydroxybenzoate 35°C Pseudomonas sp.
0.08
-
 NADH 25°C Pseudomonas sp.
0.12
-
 NADH 35°C Pseudomonas sp.
0.14
-
 NADPH 25°C Pseudomonas sp.
0.16
-
 NADPH 35°C Pseudomonas sp.
0.18
-
 NADH 45°C Pseudomonas sp.
0.2
-
 NADPH at 45°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43632
-
 2 * 43632, calculation from nucleotide sequence Pseudomonas sp.
44000
-
 2 * 44000, SDS-PAGE Pseudomonas sp.
90000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 CBS3
-
Pseudomonas sp. CBS3
-
 CBS3
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dihydroxybenzoate + NADPH + O2 8% of the activity with 4-hydroxybenzoate Pseudomonas sp. 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
 ?
2,4-dihydroxybenzoate + NADPH + O2 8% of the activity with 4-hydroxybenzoate Pseudomonas sp. CBS3 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
 ?
2-chloro-4-hydroxybenzoate + NADH + O2 40% of the activity with 4-hydroxybenzoate Pseudomonas sp. ?
-
 ?
2-chloro-4-hydroxybenzoate + NADH + O2 40% of the activity with 4-hydroxybenzoate Pseudomonas sp. CBS3 ?
-
 ?
2-fluoro-4-hydroxybenzoate + NADH + O2 50% of the activity with 4-hydroxybenzoate Pseudomonas sp. ?
-
 ?
2-fluoro-4-hydroxybenzoate + NADH + O2 50% of the activity with 4-hydroxybenzoate Pseudomonas sp. CBS3 ?
-
 ?
4-hydroxybenzoate + NADH + O2
-
 Pseudomonas sp. protocatechuate + NAD+ + H2O
-
 ?
4-hydroxybenzoate + NADH + O2
-
 Pseudomonas sp. CBS3 protocatechuate + NAD+ + H2O
-
 ?
4-hydroxybenzoate + NADPH + O2
-
 Pseudomonas sp. protocatechuate + NADP+ + H2O
-
 ?
4-hydroxybenzoate + NADPH + O2
-
 Pseudomonas sp. CBS3 protocatechuate + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 44000, SDS-PAGE Pseudomonas sp.
dimer 2 * 43632, calculation from nucleotide sequence Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.6
-
 NADPH 25°C Pseudomonas sp.
6.8
-
 NADH 25°C Pseudomonas sp.
7.4
-
 4-hydroxybenzoate 35°C Pseudomonas sp.
8.6
-
 NADPH 35°C Pseudomonas sp.
11.6
-
 NADH 35°C Pseudomonas sp.
13.3
-
 NADPH 45°C Pseudomonas sp.
16.9
-
 NADH 45°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD Km: 150 nM Pseudomonas sp.
additional information helix H2 is involved in determining the coenzyme specificity Pseudomonas sp.
NADH activity with NADH is higher than activity with NADPH Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
50
-
 Cl-
-
 Pseudomonas sp.