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Literature summary for 1.14.13.2 extracted from

  • Sterjiades, R.
    Properties of NADH/NADPH-dependent p-hydroxybenzoate hydroxylase from Moraxella sp (1993), Biotechnol. Appl. Biochem., 17, 77-90.
No PubMed abstract available
Search for more literature for 1.14.13.2

Inhibitors

Inhibitors Comment Organism Structure
3-hydroxybenzoate 2 mM, 26% inhibition Moraxella sp.
4-hydroxy-3-nitrobenzoic acid 2 mM, 23% inhibition Moraxella sp.
4-hydroxybenzaldehyde 2 mM, 27% inhibition Moraxella sp.
4-hydroxybenzoate above 0.1 mM Moraxella sp.
4-hydroxyphenylacetic acid 2 mM, 16% inhibition Moraxella sp.
4-nitrophenol 2 mM, 39% inhibition Moraxella sp.
benzoate 2 mM, 48% inhibition Moraxella sp.
coumaric acid 2 mM, 30% inhibition Moraxella sp.
Hg2+ 0.1 mM HgCl2, complete inhibition Moraxella sp.
PCMB 0.015 mM, more than 70% inhibition, partially restored by addition of 0.14 M 2-mercaptoethanol Moraxella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0095
-
 4-hydroxybenzoate reaction with NADH Moraxella sp.
0.037
-
 NADH
-
 Moraxella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, SDS-PAGE Moraxella sp.
85000
-
 gel filtration Moraxella sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxybenzoate + NADPH + O2 Moraxella sp. degradation of 4-hydroxybenzoate ?
-
 ?
4-hydroxybenzoate + NADPH + O2 Moraxella sp. GU2 degradation of 4-hydroxybenzoate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Moraxella sp.
-
-
-
Moraxella sp. GU2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Moraxella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.2
-
-
 Moraxella sp.

Storage Stability

Storage Stability Organism
-70°C, 5 mM potassium phosphate, pH 7.5, 5% glycerol, stable for several months Moraxella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzoate + NADH + O2
-
 Moraxella sp. protocatechuate + NAD+ + H2O
-
 ?
4-hydroxybenzoate + NADH + O2
-
 Moraxella sp. GU2 protocatechuate + NAD+ + H2O
-
 ?
4-hydroxybenzoate + NADPH + O2
-
 Moraxella sp. protocatechuate + NADP+ + H2O
-
 ?
4-hydroxybenzoate + NADPH + O2
-
 Moraxella sp. GU2 protocatechuate + NADP+ + H2O
-
 ?
4-hydroxybenzoate + NADPH + O2 degradation of 4-hydroxybenzoate Moraxella sp. ?
-
 ?
4-hydroxybenzoate + NADPH + O2 degradation of 4-hydroxybenzoate Moraxella sp. GU2 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE Moraxella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 pH 7.5, 20 mM potassium phosphate buffer, 20 min, stable Moraxella sp.
60
-
 in presence of 1 mM 4-hydroxybenzoate, 40% loss of activity after 20 min Moraxella sp.
65
-
 1 mM 4-hydroxybenzoate, 95% loss of activity Moraxella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.3 reaction with NADH Moraxella sp.
7.5 7.8 reaction with NADPH Moraxella sp.

Cofactor

Cofactor Comment Organism Structure
FAD contains 0.12 mol FAD per mol of enzyme Moraxella sp.
NADH activity with NADH is higher than activity with NADPH Moraxella sp.
NADPH activity with NADH is higher than activity with NADPH Moraxella sp.