Application | Comment | Organism |
---|---|---|
drug development | essential for virulence, validating this enzyme as a drug target | Aspergillus fumigatus |
General Stability | Organism |
---|---|
NADP+, and not NAD+, protects the enzyme from proteolysis | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
NADPH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus | |
0.1 | - |
NADH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NADH + H+ + O2 | Aspergillus fumigatus | first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence | N5-hydroxy-L-ornithine + NAD+ + H2O | - |
? | |
L-ornithine + NADPH + H+ + O2 | Aspergillus fumigatus | first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q5SE95 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NADH + H+ + O2 | first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NAD+ + H2O | - |
? | |
L-ornithine + NADH + H+ + O2 | accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NAD+ + H2O | - |
? | |
L-ornithine + NADPH + H+ + O2 | first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
L-ornithine + NADPH + H+ + O2 | accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SidA | - |
Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
NADPH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus | |
0.73 | - |
NADH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme | Aspergillus fumigatus | |
NADPH | accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme. NADP+, and not NAD+, protects the enzyme from proteolysis | Aspergillus fumigatus |
General Information | Comment | Organism |
---|---|---|
physiological function | first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence | Aspergillus fumigatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.3 | - |
NADH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus | |
50 | - |
NADPH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus |