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Literature summary for 1.14.13.179 extracted from

  • Summers, R.M.; Louie, T.M.; Yu, C.L.; Subramanian, M.
    Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source (2011), Microbiology, 157, 583-592.
    View publication on PubMed
Search for more literature for 1.14.13.179

Metals/Ions

Metals/Ions Comment Organism Structure
non-heme iron a non-heme iron oxygenase Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida H9N290
-
-
Pseudomonas putida CBB5 H9N290
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Reaction

Reaction Comment Organism Reaction ID
3-methylxanthine + O2 + NAD(P)H + H+ = xanthine + NAD(P)+ + H2O + formaldehyde (2) Pseudomonas putida
a
theobromine + O2 + NAD(P)H + H+ = 7-methylxanthine + NAD(P)+ + H2O + formaldehyde (1) Pseudomonas putida
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylxanthine + O2 + NAD(P)H + H+
-
 Pseudomonas putida xanthine + NAD(P)+ + H2O + formaldehyde
-
 ?
3-methylxanthine + O2 + NAD(P)H + H+
-
 Pseudomonas putida CBB5 xanthine + NAD(P)+ + H2O + formaldehyde
-
 ?
theobromine + O2 + NAD(P)H + H+
-
 Pseudomonas putida 7-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
 ?
theobromine + O2 + NAD(P)H + H+
-
 Pseudomonas putida CBB5 7-methylxanthine + NAD(P)+ + H2O + formaldehyde
-
 ?

Subunits

Subunits Comment Organism
More the soluble N-demethylase holoenzyme is composed of two components, a reductase component with cytochrome c reductase activity (Ccr) and a two-subunit N-demethylase component (Ndm). Ndm, with a native molecular mass of 240000 Da, is composed of NdmA (40000 Da) and NdmB (35000 Da). Ccr transfers reducing equivalents from NAD(P)H to Ndm, which catalyses an oxygen-dependent N-demethylation of methylxanthines to xanthine, formaldehyde and water Pseudomonas putida

Synonyms

Synonyms Comment Organism
ndmB
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH a reductase component with cytochrome c reductase activity transfers reducing equivalents from NAD(P)H to the enzyme. NADH is the preferred substrate of the reductase component. Activity with NADPH is only 22% of that with NADH Pseudomonas putida
NADPH a reductase component with cytochrome c reductase activity transfers reducing equivalents from NAD(P)H to the enzyme. NADH is the preferred substrate of the reductase component. Activity with NADPH is only 22% of that with NADH Pseudomonas putida