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Literature summary for 1.14.13.167 extracted from

  • Zhang, J.J.; Liu, H.; Xiao, Y.; Zhang, X.E.; Zhou, N.Y.
    Identification and characterization of catabolic para-nitrophenol 4-monooxygenase and para-benzoquinone reductase from Pseudomonas sp. strain WBC-3 (2009), J. Bacteriol., 191, 2703-2710.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.167

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 4-nitrophenol His-tagged enzyme, in 20 mM phosphate buffer (pH 7.0), temperature not specified in the publication Pseudomonas sp.
0.1374
-
 NADPH His-tagged enzyme, in 20 mM phosphate buffer (pH 7.0), temperature not specified in the publication Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information addition of Mg2+ or Mn2+ ions has no effect on the activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 1 * 47000, His-tagged enzyme, gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. C1I201
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:4-nitrophenol-grown cell
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
 crude extract, at pH 7.0 and 30°C Pseudomonas sp.
6.8
-
 purified enzyme, at pH 7.0 and 30°C Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, without glycerol, rapid inactivation Pseudomonas sp.
-70°C, in 50% (v/v) glycerol, 7 days, 70% loss of activity Pseudomonas sp.
0°C, storage medium, no loss of activity after several hours, 30% loss of activity after 12 h, 80% loss of activity after 40 h Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrocatechol + NADPH + H+ + O2 lower activity compared to 4-nitrophenol Pseudomonas sp. ?
-
 ?
4-nitrophenol + NADPH + H+ + O2 monooxygenation Pseudomonas sp. 4-benzoquinone + nitrite + NADP+ + H2O
-
 ?
additional information no activity with 3-nitrophenol, 2-nitrophenol, 4-nitrobenzoate, nitrobenzene, 3-dinitrobenzene, 2,6-dinitrotoluene, 3-hydroxy-4-nitrobenzoate, 2,4,6-trinitrophenol, and 4-chlorophenol Pseudomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 47000, His-tagged enzyme, gel filtration Pseudomonas sp.

Synonyms

Synonyms Comment Organism
para-nitrophenol 4-monooxygenase
-
 Pseudomonas sp.
PNP 4-monooxygenase
-
 Pseudomonas sp.
pnpA
-
 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD dependent on, negligible activity is observed when FMN replaces FAD Pseudomonas sp.
NADH NADPH can be replaced by NADH with the same efficiency Pseudomonas sp.
NADPH
-
 Pseudomonas sp.

Expression

Organism Comment Expression
Pseudomonas sp. 0.0018 mg/ml of para-benzoquinone reductase PnpB enhances the 4-nitrophenol degradation activity by para-nitrophenol 4-monooxygenase by about 2fold up

General Information

General Information Comment Organism
physiological function para-nitrophenol 4-monooxygenase gene pnpA plays an essential role in 4-nitrophenol mineralization in strain Pseudomonas putida strain WBC-3 Pseudomonas sp.