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Literature summary for 1.14.13.167 extracted from

  • Kitagawa, W.; Kimura, N.; Kamagata, Y.
    A novel p-nitrophenol degradation gene cluster from a gram-positive bacterium, Rhodococcus opacus SAO101 (2004), J. Bacteriol., 186, 4894-4902.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.167

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3)pLysS cells Rhodococcus opacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59700
-
 x * 59700, calculated from amino acid sequence Rhodococcus opacus

Organism

Organism UniProt Comment Textmining
Rhodococcus opacus
-
-
-
Rhodococcus opacus SAO101
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:4-nitrophenol-grown cell
-
 Rhodococcus opacus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000046
-
 using 2,4,6-trinitrocatechol as substrate, pH and temperature not specified in the publication Rhodococcus opacus
0.000025
-
 using 4-nitrocatechol as substrate, pH and temperature not specified in the publication Rhodococcus opacus
0.0000264
-
 using 4-nitrophenol as substrate, pH and temperature not specified in the publication Rhodococcus opacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4,6-trinitrophenol + NADH + H+ + O2 17% activity compared to 4-nitrophenol Rhodococcus opacus ?
-
 ?
2,4,6-trinitrophenol + NADH + H+ + O2 17% activity compared to 4-nitrophenol Rhodococcus opacus SAO101 ?
-
 ?
4-nitrocatechol + NADH + H+ + O2 96% activity compared to 4-nitrophenol Rhodococcus opacus 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O
-
 ?
4-nitrocatechol + NADH + H+ + O2 96% activity compared to 4-nitrophenol Rhodococcus opacus SAO101 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O
-
 ?
4-nitrophenol + NADH + H+ + O2 100% activity Rhodococcus opacus ?
-
 ?
4-nitrophenol + NADH + H+ + O2 100% activity Rhodococcus opacus SAO101 ?
-
 ?
additional information almost no activity towards 2,4,6-trinitrophenol Rhodococcus opacus ?
-
 ?
additional information almost no activity towards 2,4,6-trinitrophenol Rhodococcus opacus SAO101 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 59700, calculated from amino acid sequence Rhodococcus opacus

Synonyms

Synonyms Comment Organism
4-NP/4-NCA monooxygenase
-
 Rhodococcus opacus
NpcA
-
 Rhodococcus opacus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Rhodococcus opacus

General Information

General Information Comment Organism
malfunction the npcA mutant strain SDA1 completely loses the ability to grow on 4-nitrophenol as the sole carbon source Rhodococcus opacus