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Literature summary for 1.14.13.166 extracted from

  • Kadiyala, V.; Spain, J.C.
    A two-component monooxygenase catalyzes both the hydroxylation of p-nitrophenol and the oxidative release of nitrite from 4-nitrocatechol in Bacillus sphaericus JS905 (1998), Appl. Environ. Microbiol., 64, 2479-2484.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.166

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Lysinibacillus sphaericus
Methimazole 58% inhibition at 0.5 mM Lysinibacillus sphaericus
additional information alpha-naphthoflavone, miconazole, and metyrapone at 0.5 mM have very little effect (less than 5% inhibition) on enzyme activity Lysinibacillus sphaericus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ magnesium ions enhance the enzyme activity Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-
Lysinibacillus sphaericus JS905
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephacryl S-300 gel filtration Lysinibacillus sphaericus

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:4-nitrophenol-grown cell
-
 Lysinibacillus sphaericus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0013
-
 cell extract, using 4-nitrocatechol as substrate, at pH 8.0 and 30°C Lysinibacillus sphaericus
0.0013
-
 cell extract, using 4-nitrophenol as substrate, at pH 8.0 and 30°C Lysinibacillus sphaericus
0.038
-
 after 28fold purification, using 4-nitrocatechol as substrate, at pH 8.0 and 30°C Lysinibacillus sphaericus
0.04
-
 after 32fold purification, using 4-nitrophenol as substrate, at pH 8.0 and 30°C Lysinibacillus sphaericus

Storage Stability

Storage Stability Organism
. Lysinibacillus sphaericus
0°C, extracts prepared without added FAD, 48 h, 52% loss of activity Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrocatechol + NADH + H+ + O2
-
 Lysinibacillus sphaericus 2-hydroxy-1,4-benzoquinone + nitrite + NAD+ + H2O
-
 ?
additional information NADPH does not support 4-nitrophenol or 4-nitrocatechol oxidation Lysinibacillus sphaericus ?
-
 ?
additional information NADPH does not support 4-nitrophenol or 4-nitrocatechol oxidation Lysinibacillus sphaericus JS905 ?
-
 ?

Synonyms

Synonyms Comment Organism
PNP monooxygenase the enzyme system consists of two components that catalyze two consecutive monooxygenation reactions Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in 50 mM Tris-HCl Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
FAD required for full activity. The reductase component of the enzyme has a loosely bound FAD Lysinibacillus sphaericus